Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants

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dc.contributor.authorWang L.
dc.contributor.authorKaya H.B.
dc.contributor.authorZhang N.
dc.contributor.authorRai R.
dc.contributor.authorWillmann M.R.
dc.contributor.authorCarpenter S.C.D.
dc.contributor.authorRead A.C.
dc.contributor.authorMartin F.
dc.contributor.authorFei Z.
dc.contributor.authorLeach J.E.
dc.contributor.authorMartin G.B.
dc.contributor.authorBogdanove A.J.
dc.date.accessioned2024-07-22T08:06:40Z
dc.date.available2024-07-22T08:06:40Z
dc.date.issued2021
dc.description.abstractPrime editing is an adaptation of the CRISPR-Cas system that uses a Cas9(H840A)-reverse transcriptase fusion and a guide RNA amended with template and primer binding site sequences to achieve RNA-templated conversion of the target DNA, allowing specified substitutions, insertions, and deletions. In the first report of prime editing in plants, a variety of edits in rice and wheat were described, including insertions up to 15 bp. Several studies in rice quickly followed, but none reported a larger insertion. Here, we report easy-to-use vectors for prime editing in dicots as well as monocots, their validation in Nicotiana benthamiana, rice, and Arabidopsis, and an insertion of 66 bp that enabled split-GFP fluorescent tagging. Copyright © 2021 Wang, Kaya, Zhang, Rai, Willmann, Carpenter, Read, Martin, Fei, Leach, Martin and Bogdanove.
dc.identifier.DOI-ID10.3389/fgeed.2021.617553
dc.identifier.issn26733439
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13655
dc.language.isoEnglish
dc.publisherFrontiers Media S.A.
dc.rightsAll Open Access; Gold Open Access
dc.titleSpelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants
dc.typeArticle

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