Molecular prevalence of Blastocystis sp. and subtype diversity in fecal samples collected from cattle in dairy farms in Turkey

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dc.contributor.authorÖncü Öner T.
dc.contributor.authorKarakavuk M.
dc.contributor.authorDeğirmenci Döşkaya A.
dc.contributor.authorGüvendi M.
dc.contributor.authorGül A.
dc.contributor.authorKöseoğlu A.E.
dc.contributor.authorErkunt Alak S.
dc.contributor.authorGürüz A.Y.
dc.contributor.authorÜn C.
dc.contributor.authorDöşkaya M.
dc.contributor.authorCan H.
dc.date.accessioned2024-07-22T08:04:17Z
dc.date.available2024-07-22T08:04:17Z
dc.date.issued2022
dc.description.abstractClose contact with infected animals is one of the main risk factors for zoonotic transmission of enteric protozoan parasite Blastocystis and thus, several animal species are being screened for the detection of the zoonotic subtypes. For this purpose, 22 fecal samples were collected from healthy cattle aged > 2 months and 39 fecal samples were also collected from cattle (aged <2 months) which are treated with TMP-SMX due to diarrhea. Later, Blastocystis sp. and subtypes were investigated by a PCR targeting the SSU rRNA gene and subsequently by sequencing. Among the 22 fecal samples collected from healthy cattle, Blastocystis was detected in 12 of them with a prevalence rate of 54.5 %. Among Blastocystis-positive samples, five different subtypes (ST3, ST5, ST10, ST12, and ST13) were detected. The predominant subtype was ST10 (allele 152) with a prevalence rate of 50 % (6/12). In the other group treated with TMP-SMX due to diarrhea, Blastocystis was detected in only one (2.56 %;1/39) fecal sample and its subtype was ST1 (allele 2). High prevalence of Blastocystis as well as predominance of ST10 (allele 152) were detected in healthy cattle. The identification of zoonotic ST1, ST3, ST5 and ST12 subtypes among the detected subtypes with a high prevalence (46.1 %; 6/13) showed the importance of cattle as a source for transmission of Blastocystis to humans. It was observed that the efficiency of TMP-SMX on the clearance of Blastocystis in cattle was very strong. Moreover, to our knowledge, this is the first study detecting Blastocystis ST13 subtype in the cattle. © 2022 Elsevier Ltd
dc.identifier.DOI-ID10.1016/j.cimid.2022.101850
dc.identifier.issn01479571
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12643
dc.language.isoEnglish
dc.publisherElsevier Ltd
dc.subjectAnimals
dc.subjectBlastocystis
dc.subjectBlastocystis Infections
dc.subjectCattle
dc.subjectCattle Diseases
dc.subjectDiarrhea
dc.subjectFarms
dc.subjectFeces
dc.subjectGenetic Variation
dc.subjectHumans
dc.subjectPhylogeny
dc.subjectPrevalence
dc.subjectTrimethoprim, Sulfamethoxazole Drug Combination
dc.subjectTurkey
dc.subjectcotrimoxazole
dc.subjectmetronidazole
dc.subjectnitazoxanide
dc.subjectparomomycin
dc.subjectcotrimoxazole
dc.subjectallele
dc.subjectanimal experiment
dc.subjectArticle
dc.subjectBlastocystis
dc.subjectcontrolled study
dc.subjectdairy cattle
dc.subjectdiarrhea
dc.subjectDNA extraction
dc.subjectDNA isolation
dc.subjectfeces analysis
dc.subjectmicrobial diversity
dc.subjectnonhuman
dc.subjectpolymerase chain reaction
dc.subjectprevalence
dc.subjectST10 allele 152
dc.subjectST12
dc.subjectST13
dc.subjectST3
dc.subjectST5
dc.subjectzoonotic transmission
dc.subjectagricultural land
dc.subjectanimal
dc.subjectblastocystosis
dc.subjectbovine
dc.subjectcattle disease
dc.subjectdiarrhea
dc.subjectfeces
dc.subjectgenetic variation
dc.subjectgenetics
dc.subjecthuman
dc.subjectparasitology
dc.subjectphylogeny
dc.subjectprevalence
dc.subjectturkey (bird)
dc.subjectveterinary medicine
dc.titleMolecular prevalence of Blastocystis sp. and subtype diversity in fecal samples collected from cattle in dairy farms in Turkey
dc.typeArticle

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