Simultaneous analysis of biochemical markers in vitreous humour and serum: A preliminary study on the effect of storage time at -20°C

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dc.contributor.authorGurler M.
dc.contributor.authorOzturk G.
dc.contributor.authorKir M.Z.
dc.contributor.authorGinis Z.
dc.contributor.authorErden G.
dc.contributor.authorAkyol S.
dc.contributor.authorKaya M.
dc.contributor.authorKarapirli M.
dc.contributor.authorAkyol O.
dc.date.accessioned2024-07-22T08:12:13Z
dc.date.available2024-07-22T08:12:13Z
dc.date.issued2016
dc.description.abstractVitreous humour (VH) is a quite stable and well-protected biological fluid from post-mortem degradation and contamination, making it a usable specimen in forensic pathology. We aimed to analyse the biochemical markers in VH stored for one month at -20°C and to compare them with those of serum samples of the same deceased people. To the best of our knowledge this is the first time this has been reported in the literature. Serum levels of sodium, potassium and calcium, phosphorous and VH levels of calcium, creatinine, and urea were not statistically different between fresh and stored samples. There was no significant difference in VH urea and creatinine levels whereas serum levels were significantly decreased by the 15th and 30th days. In conclusion, urea and creatinine levels in VH showed adequate stability after one month of storage at -20°C, whereas large variations were seen in the stability of inorganic components such as Na, K, Mg, Cl, and P. Our results indicate the validity of using post-mortem VH samples for urea and creatinine but not for VH stored at -20°C for a limited time frame. The differences in sample handling before being stored at -20°C may introduce bias on some components of VH. © 2015 Australian Academy of Forensic Sciences.
dc.identifier.DOI-ID10.1080/00450618.2015.1042046
dc.identifier.issn00450618
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15971
dc.language.isoEnglish
dc.publisherTaylor and Francis Ltd.
dc.subjectbiochemical marker
dc.subjectcalcium
dc.subjectchloride
dc.subjectchloride ion
dc.subjectcreatinine
dc.subjectmagnesium
dc.subjectnitrogen
dc.subjectphosphorus
dc.subjectpotassium
dc.subjectsodium
dc.subjecturea
dc.subjectadolescent
dc.subjectadult
dc.subjectaged
dc.subjectaqueous humor
dc.subjectArticle
dc.subjectautopsy
dc.subjectcalcium blood level
dc.subjectchild
dc.subjectchloride blood level
dc.subjectclinical article
dc.subjectcontrolled study
dc.subjectcreatinine blood level
dc.subjectfemale
dc.subjecthuman
dc.subjectmagnesium blood level
dc.subjectmale
dc.subjectphosphate blood level
dc.subjectpotassium blood level
dc.subjectsodium blood level
dc.subjectstorage temperature
dc.subjecturea nitrogen blood level
dc.subjectvitreous body
dc.titleSimultaneous analysis of biochemical markers in vitreous humour and serum: A preliminary study on the effect of storage time at -20°C
dc.typeArticle

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