Intraoperative evaluation of testicular vascularization and perfusion in rat testicles with indocyanine green (ICG)/near-infrared (NIR) fluorescent imaging after torsion–detorsion and reperfusion

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dc.contributor.authorŞencan A.
dc.contributor.authorTanrıverdi H.İ.
dc.contributor.authorŞimşek F.B.
dc.contributor.authorUsta İ.B.
dc.contributor.authorÜçöz M.
dc.contributor.authorÖzbilgin K.
dc.date.accessioned2024-07-22T08:04:02Z
dc.date.available2024-07-22T08:04:02Z
dc.date.issued2022
dc.description.abstractIntroduction: The aim of this study was to evaluate testicular perfusion and vascularization with intraoperative ICG/NIR imaging in a testicular ischemia–reperfusion model and to investigate the effects of ICG on testicular tissue. Materials and methods: 24 male rats were divided into four groups. In the ICG group, only ICG was given and images of the testicles were recorded with NIR camera. In the torsion group, the testicles were left in torsion for 4 h. ICG/NIR images were obtained after torsion and detorsion. In the reperfusion group, ICG/NIR images of the testicles were obtained at the 4th hour of reperfusion. After the procedures, testicles were collected and evaluated with histological, immunohistochemical examination and qRT-PCR. Results: There was no histologically negative effect of ICG on testicular tissue. There was no testicular perfusion in the torsion group, but perfusion started after detorsion. At the 4th hour of reperfusion, testicular perfusion continued. TNF-a, IL-6, MCP-1 and caspase-3 immunoreactivity were found to be at low levels in the control and ICG groups, while high in the torsion and reperfusion groups (p < 0.05). In qRT-PCR, TNF-a, IL-6, MCP-1 and caspase-3 expressions were lower in the control and ICG groups, but higher in the torsion and reperfusion groups. Conclusion: There was no histologically negative effect of ICG on testicles. The ICG/NIR imaging technique seems to be a feasible method in testicular torsion and may contribute to the surgeon in the intraoperative management of testicular torsion. In testicles that started to be perfused after detorsion, perfusion still continued at the 4th hour of reperfusion. Our next goal is to test whether testicles showing ICG fluorescence in during reperfusion maintain their viability for long term. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
dc.identifier.DOI-ID10.1007/s00383-022-05211-1
dc.identifier.issn01790358
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12543
dc.language.isoEnglish
dc.publisherSpringer Science and Business Media Deutschland GmbH
dc.subjectAnimals
dc.subjectCaspase 3
dc.subjectHumans
dc.subjectIndocyanine Green
dc.subjectInterleukin-6
dc.subjectMale
dc.subjectRats
dc.subjectReperfusion
dc.subjectReperfusion Injury
dc.subjectSpermatic Cord Torsion
dc.subjectTestis
dc.subjectcaspase 3
dc.subjecticg pulsion
dc.subjectindocyanine green
dc.subjectinterleukin 6
dc.subjectmonocyte chemotactic protein 1
dc.subjectoligonucleotide
dc.subjecttumor necrosis factor
dc.subjectxylazine
dc.subjectcaspase 3
dc.subjectindocyanine green
dc.subjectinterleukin 6
dc.subjectadult
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectcontrolled study
dc.subjectdrug effect
dc.subjectfluorescence imaging
dc.subjectgene
dc.subjecthistopathology
dc.subjectimmunohistochemistry
dc.subjectimmunoreactivity
dc.subjectintraoperative period
dc.subjectmale
dc.subjectnear infrared spectroscopy
dc.subjectnonhuman
dc.subjectorgan perfusion
dc.subjectprotein expression
dc.subjectrat
dc.subjectreal time polymerase chain reaction
dc.subjectreperfusion injury
dc.subjectstaining
dc.subjecttestis tissue
dc.subjecttestis torsion
dc.subjectvascularization
dc.subjectyoung adult
dc.subjectanimal
dc.subjectdiagnostic imaging
dc.subjecthuman
dc.subjectreperfusion
dc.subjecttestis
dc.subjecttestis torsion
dc.titleIntraoperative evaluation of testicular vascularization and perfusion in rat testicles with indocyanine green (ICG)/near-infrared (NIR) fluorescent imaging after torsion–detorsion and reperfusion
dc.typeArticle

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