Examination of bcl-2 and p53 expressions and apoptotic index by TUNEL method in psoriasis

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dc.contributor.authorGündüz K.
dc.contributor.authorDemireli P.
dc.contributor.authorVatansever S.
dc.contributor.authorInanir I.
dc.date.accessioned2024-07-22T08:23:08Z
dc.date.available2024-07-22T08:23:08Z
dc.date.issued2006
dc.description.abstractBackground: Psoriasis is characterised by hyperproliferation and by aberrant differentiation. Blockage of the normal apoptotic process is one of the factors implicated in the pathogenesis. Objective: To determine the apoptotic features by using TUNEL method and also bcl-2 and p53 expressions in psoriatic epidermis. Materials and methods: Biopsies of 35 patients with psoriasis vulgaris and 14 normal skin were evaluated. Apoptotic cells were detected using the dUTP nick-end labelling assay; bc1-2 and p53 expressions were assessed by using immunohistochemical techniques. A semi-quantitative grading system (HSCORE) was used for comparison. Results: Bcl-2 was strongly expressed in basal keratinocytes of normal skin, while no expression was observed in 13 (37.2%) of the psoriatic samples and it was weakly expressed in the spinous cell layer of 22 (62.8%) samples. Moderate p53 expression was observed in the psoriasis group, while it was weak in the control. The percentage of TUNEL positive cells were significantly increased in the psoriasis group (65±2.30) when compared with the control (32.84±7.16). Conclusion: Apoptotic index besides bc1-2 and p53 expressions in psoriasis differ from normal epidermis. Down-regulation of bc1-2 is consistent with the dynamics of psoriasis but increased TUNEL positive cells and p53 expression has not been fully elucidated yet. © Blackwell Munksgaard 2006.
dc.identifier.DOI-ID10.1111/j.1600-0560.2006.00564.x
dc.identifier.issn16000560
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19374
dc.language.isoEnglish
dc.subjectApoptosis
dc.subjectDown-Regulation
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectIn Situ Nick-End Labeling
dc.subjectProto-Oncogene Proteins c-bcl-2
dc.subjectPsoriasis
dc.subjectTumor Suppressor Protein p53
dc.subjectprotein bcl 2
dc.subjectprotein p53
dc.subjectapoptosis
dc.subjectarticle
dc.subjectcell differentiation
dc.subjectcell proliferation
dc.subjectclinical feature
dc.subjectcontrolled study
dc.subjectdown regulation
dc.subjectepidermis
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectkeratinocyte
dc.subjectnick end labeling
dc.subjectpathogenesis
dc.subjectprotein expression
dc.subjectpsoriasis
dc.subjectpsoriasis vulgaris
dc.subjectquantitative analysis
dc.subjectskin biopsy
dc.titleExamination of bcl-2 and p53 expressions and apoptotic index by TUNEL method in psoriasis
dc.typeArticle

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