Immunolocalizations of VEGF, its receptors flt-1, KDR and TGF-β's in epithelial ovarian tumors

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dc.contributor.authorInan S.
dc.contributor.authorVatansever S.
dc.contributor.authorCelik-Ozenci C.
dc.contributor.authorSanci M.
dc.contributor.authorDicle N.
dc.contributor.authorDemir R.
dc.date.accessioned2024-07-22T08:23:11Z
dc.date.available2024-07-22T08:23:11Z
dc.date.issued2006
dc.description.abstractObjective: Angiogenesis is an essential factor for growth, differentiation, invasion and metastasis of tumors. In this study, we aimed to evaluate the immunolocalizations of vascular endothelial growth factor (VEGF), its receptors flt-1, KDR/flk-1, and transforming growth factor-beta's (TGF-β) in epithelial ovarian tumors, utilizing indirect immunohistochemistry to understand the role of the angiogenic events in ovarian neoplasia. Methods: Tissue blocks from 40 patients who had ovarian pathology (borderline serous-mucinous tumor and malignant serous-mucinous adenocarcinoma of the ovary) were included in this study. All formalin-fixed, paraffin-embedded tissue sections were stained with hematoxylin-eosin or primary antibodies against VEGF, flt-1, KDR/flk-1, TGF-β1, TGF-β2 and TGF-β3 using the avidin-biotin-peroxidase method. H-SCORE, a semi-quantitative grading system, was used to compare immunohistochemical staining intensities. Results: Positive VEGF immunoreactivity was concentrated in the epithelial and stromal parts of all the ovarian samples and the endothelial cells in the stroma were also stained. Increased immunoreactivity of VEGF was observed in malignant ovarian adenocarcinomas compared to the borderline tumors of the ovary. VEGF receptors, flt-1 and KDR/flk-1 immunoreactivities were detected not only in vascular endothelial cells, but also in tumor cells at malignant sites. Immunoreactivities of VEGF and its receptors were coexpressed in tumor cells of the ovarian carcinoma. While immunoreactivities of TGF-β1 and TGF-β2 were both overexpressed in malignant ovarian carcinomas, immunoreactivity of TGF-β3 was still mild. Conclusion: Our results suggest that overexpression of VEGF, its receptors flt-1, KDR/flk-1 and TGF-β interaction may play an important role in the ovarian cancer biology, with potential effects on tumor growth and angiogenesis. New therapeutic strategies using VEGF and TGF-β antagonists could obtain an additional approach to the treatment ovarian carcinoma by inhibiting angiogenesis.
dc.identifier.issn02133911
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19416
dc.language.isoEnglish
dc.subjectAdult
dc.subjectAged
dc.subjectFemale
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectMiddle Aged
dc.subjectNeovascularization, Pathologic
dc.subjectOvarian Neoplasms
dc.subjectOvary
dc.subjectTransforming Growth Factor beta
dc.subjectVascular Endothelial Growth Factor A
dc.subjectVascular Endothelial Growth Factor Receptor-1
dc.subjectVascular Endothelial Growth Factor Receptor-2
dc.subjectavidin
dc.subjectbiotin
dc.subjecteosin
dc.subjectformaldehyde
dc.subjecthematoxylin
dc.subjectmonoclonal antibody
dc.subjectparaffin
dc.subjectperoxidase
dc.subjecttransforming growth factor beta receptor
dc.subjecttransforming growth factor beta1
dc.subjecttransforming growth factor beta2
dc.subjecttransforming growth factor beta3
dc.subjectvasculotropin
dc.subjectvasculotropin receptor 1
dc.subjectvasculotropin receptor 2
dc.subjectFLT1 protein, human
dc.subjecttransforming growth factor beta
dc.subjectvasculotropin A
dc.subjectvasculotropin receptor 1
dc.subjectvasculotropin receptor 2
dc.subjectadult
dc.subjectaged
dc.subjectangiogenesis
dc.subjectarticle
dc.subjectcancer invasion
dc.subjectcarcinogenesis
dc.subjectclinical article
dc.subjectcontrolled study
dc.subjectdrug targeting
dc.subjectendothelium cell
dc.subjectfemale
dc.subjectgene overexpression
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectimmunolocalization
dc.subjectimmunoreactivity
dc.subjectlaparotomy
dc.subjectmetastasis potential
dc.subjectmucinous carcinoma
dc.subjectovary adenocarcinoma
dc.subjectprotein interaction
dc.subjectquantitative analysis
dc.subjecttissue fixation
dc.subjecttissue section
dc.subjecttumor cell
dc.subjecttumor differentiation
dc.subjecttumor growth
dc.subjectvascular endothelium
dc.subjectgene expression regulation
dc.subjectimmunohistochemistry
dc.subjectmetabolism
dc.subjectmethodology
dc.subjectmiddle aged
dc.subjectneovascularization (pathology)
dc.subjectovary
dc.subjectovary tumor
dc.subjectpathology
dc.titleImmunolocalizations of VEGF, its receptors flt-1, KDR and TGF-β's in epithelial ovarian tumors
dc.typeArticle

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