Investigation of phenolic content in five different pine barks species grown in Turkey by HPLC-UV and LC-MS

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dc.contributor.authorŞeker M.E.
dc.contributor.authorÇelik A.
dc.contributor.authorDost K.
dc.contributor.authorErdoğan A.
dc.date.accessioned2024-07-22T08:06:00Z
dc.date.available2024-07-22T08:06:00Z
dc.date.issued2021
dc.description.abstractInvestigation of phenolic content from different pine bark species grown in Turkey was performed using a reversed-phase high pressure liquid chromatography with ultraviolet (RP-HPLC-UV) method. All phenolic constituents were separated in <26 min on reversed-phase C18 column with gradient mobile phase that consists of orthophosphoric acid, methanol and acetonitrile. Detections were made on an UV detector at 280 nm and at a flow rate of 1 mL/min. Samples were prepared according to Masqueller's conventional sample preparation method with slight modifications. To avoid the reduction in extraction efficiency the sample preparation step was carried out under argon atmosphere. The linearity of the method was between 0.9994 and 0.9999. The detection limits for the five phenolic constituents ranged from 0122 to 0.324 mg/L. Catechin and taxifolin were found in all pine barks at a concentration of 0.065 ± 0.002-1.454 ± 0.004 and 0.015 ± 0.001-23.164 ± 0.322 mg/g, respectively. Epicatechin was determined in four pine barks between 0.027 ± 0.001 and 0.076 ± 0.002 mg/g, ferulic acid in two pine barks between 0.010 ± 0.001 and 0.022 ± 0.001 mg/g and epicatechin gallate in only one of the pine barks at 0.025 ± 0.001 mg/g. Finally, the total amount of phenolic compounds and antioxidant capacities of the pine barks were found to be very high. © The Author(s) 2021. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com
dc.identifier.DOI-ID10.1093/chromsci/bmab022
dc.identifier.issn00219665
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13340
dc.language.isoEnglish
dc.publisherOxford University Press
dc.subjectCatechin
dc.subjectChromatography, High Pressure Liquid
dc.subjectChromatography, Reverse-Phase
dc.subjectPhenols
dc.subjectPinus
dc.subjectPlant Bark
dc.subjectQuercetin
dc.subjectTandem Mass Spectrometry
dc.subjectcatechin
dc.subjectphenol derivative
dc.subjectquercetin
dc.subjecttaxifolin
dc.subjectbark
dc.subjectchemistry
dc.subjecthigh performance liquid chromatography
dc.subjectpine
dc.subjectprocedures
dc.subjectreversed phase liquid chromatography
dc.subjecttandem mass spectrometry
dc.titleInvestigation of phenolic content in five different pine barks species grown in Turkey by HPLC-UV and LC-MS
dc.typeArticle

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