The glycoconjugate changes of apoptotic skeletal muscle tissues in regressing Eurasian green toad, Bufo viridis (Amphibia: Anura) tadpole tail
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Date
2006
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Abstract
In the present study, programmed cell death of skeletal muscle tissues of the regressing tail of Euroasian green toad Bufo viridis (Amphibia: Anura) tadpole was investigated with Hematoxylin-eosin (H+E) and TUNEL methods in the three groups of different tail lengths. TUNEL stainings indicate that, during the tail regression, apoptotic skeletal muscle tissue constitutes fragmentations and the progression of cell death occurs from the tip and outer of the tail to its base. In addition, muscle cells apoptosis occurs first near the subepidermal fibrolast layer proposed that these cells may interfere the skeletal muscle cells apoptosis during the tail regression. When the of the glycosaminoglycan alterations of these different sizes of shortening tail was analyzed histochemieally. After the Alcian Blue-Periodic Acid Schiff's (AB-PAS) sequences at critical electrolyte concentrations and different pH values, we observed carboxylated glycosaminoglycans (i.e., hyaluronic acid, HA) are increased in apoptotic muscle cells throughout the tail regression. We concluded that carboxylated glycosaminglycans may play an important role in shortening of the tail via muscle cell apoptosis. © 2006 Asian Network for Scientific Information.
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Amphibia , Animalia , Anura , Bufo debilis , Bufo viridis , alcian blue , electrolyte , eosin , glycoconjugate , glycosaminoglycan , hematoxylin , hyaluronic acid , animal tissue , apoptosis , article , carboxylation , cell interaction , concentration (parameters) , controlled study , epidermis , fibroblast , histochemistry , nick end labeling , nonhuman , periodic acid Schiff stain , pH , skeletal muscle , tadpole , tail , tissue level , toad