Effects of high doses of dexamethasone on hemodynamic and immunohistochemical characteristics of acute paraquat intoxication in rat kidneys

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dc.contributor.authorEkerbicer N.
dc.contributor.authorGurpinar T.
dc.contributor.authorTarakci F.
dc.contributor.authorTurkoz Uluer E.
dc.contributor.authorInan S.
dc.date.accessioned2024-07-22T08:11:51Z
dc.date.available2024-07-22T08:11:51Z
dc.date.issued2016
dc.description.abstractParaquat (1,1-dimethyl-4,4-bipyridinium) (PQ), is a nonselective contact herbicide that is highly toxic to humans. The kidney is affected during PQ intoxication. Dexamethasone (Dexa) has anti-inflammatory effects and is used to treat cases of PQ poisoning. We investigated in rat kidney hemodynamic effects and immunohistochemical characteristics of Dexa treatment in acute PQ poisoning. Adult male rats were divided into four groups: 1, untreated control; 2, treated with 100 mg/kg Dexa; 3, treated with 25 mg/kg PQ; 4, treated with PQ + Dexa. Mean arterial pressure (MAP) and heart rate (HR) were recorded during the experimental period (2 h). Tissues were removed after 2 h and immunohistochemistry was performed after 24 h. Paraffin sections of kidney were prepared and anti-cyclo-oxygenase-1 (COX-1), anti-cyclo-oxygenase-2 (COX-2), anti-angiotensin converting enzyme (ACE), anti-aquaporin-1 (AQU-1), anti-vascular cell adhesion molecule (VCAM) primary antibodies were used for immunohistochemical examination. Immunoreactivities were scored as: (1) minimal, (2) weak, (3) mild, (4) moderate, (5) strong and (6) very strong. MAP and HR were measured at 10 min, 20 min, 1 h and 2 h. MAP at 10 and 20 min and 1 h was increased in the Dexa group. HR also was increased in all groups compared to controls at 2 h. Compared to groups 2 and 4, MAP values decreased significantly in group 3 at 1 h. The intensity of all of immunoreactivities was decreased in group 2. In group 3, immunoreactivities of COX-1, COX-2 and ACE were decreased compared to the control and the other groups, whereas AQU-1 and VCAM immunoreactivities were the same as the control group. ACE and VCAM immunoreactivities were decreased in group 4 compared to the control group, while COX-1, COX-2 and AQU-1 immunoreactivities were close to those of the control group. Dexa appears to be useful for treating PQ intoxication. © 2016 The Biological Stain Commission.
dc.identifier.DOI-ID10.3109/10520295.2015.1109141
dc.identifier.issn10520295
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15792
dc.language.isoEnglish
dc.publisherTaylor and Francis Ltd
dc.subjectAnimals
dc.subjectAnti-Inflammatory Agents
dc.subjectDexamethasone
dc.subjectDose-Response Relationship, Drug
dc.subjectHemodynamics
dc.subjectImmunohistochemistry
dc.subjectKidney Cortex
dc.subjectMale
dc.subjectParaquat
dc.subjectRats
dc.subjectantiinflammatory agent
dc.subjectdexamethasone
dc.subjectparaquat
dc.subjectanimal
dc.subjectdose response
dc.subjectdrug effects
dc.subjecthemodynamics
dc.subjectimmunohistochemistry
dc.subjectkidney cortex
dc.subjectmale
dc.subjectrat
dc.titleEffects of high doses of dexamethasone on hemodynamic and immunohistochemical characteristics of acute paraquat intoxication in rat kidneys
dc.typeArticle

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