Immunohistochemical and ultrastructural analysis of the effect of omega-3 on embryonic implantation in an experimental mouse model

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dc.contributor.authorSarsmaz K.
dc.contributor.authorGoker A.
dc.contributor.authorMicili S.C.
dc.contributor.authorErgur B.U.
dc.contributor.authorKuscu N.K.
dc.date.accessioned2024-07-22T08:11:56Z
dc.date.available2024-07-22T08:11:56Z
dc.date.issued2016
dc.description.abstractObjective: Implantation is the first step to a healthy pregnancy. Omega-3 supplementation is common to use during pregnancy, for its antioxidant and membrane stabilising effect. In this study we have aimed to study the effect of Omega-3 supplementation on implantation in a mouse model by immunohistochemical methods and electron microscopic evaluation. Materials and methods: Mice were randomized into three groups to receive standard food, Omega-3 400 mg/kg and Omega-3 1000 mg/kg one menstrual cycle before mating. Mice were sacrificed on third day of estimated implantation and uterine horns were evaluated immunohistochemically for staining of Laminin and Leukemia Inhibitory Factor (LIF) and ultrastructural morphology. Results: Laminin and LIF immunoreactivity were increased signifcantly in the high dose group when compared to the control and low-dose groups in lumen epithelium basal membrane, gland epithelium basal membrane and endometrial stroma. Electron-microscopic evaluation showed a decrease in epithelial height and microvilli loss in the high dose groups. Conclusion: Omega-3 supplementation increased implantation markers Laminin and LIF and decreased epithelial height and microvilli thus seems to prepare the endometrium for a favorable environment of implantation. © 2016.
dc.identifier.DOI-ID10.1016/j.tjog.2016.04.011
dc.identifier.issn10284559
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15850
dc.language.isoEnglish
dc.publisherElsevier Ltd
dc.rightsAll Open Access; Gold Open Access
dc.subjectAnimals
dc.subjectEmbryo Implantation
dc.subjectEpithelial Cells
dc.subjectEpithelium
dc.subjectFatty Acids, Omega-3
dc.subjectFemale
dc.subjectLaminin
dc.subjectLeukemia Inhibitory Factor
dc.subjectMice
dc.subjectMicroscopy, Electron, Transmission
dc.subjectMicrovilli
dc.subjectModels, Animal
dc.subjectPregnancy
dc.subjectUterus
dc.subjectlaminin
dc.subjectleukemia inhibitory factor
dc.subjectomega 3 fatty acid
dc.subjectlaminin
dc.subjectleukemia inhibitory factor
dc.subjectLif protein, mouse
dc.subjectomega 3 fatty acid
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectbasement membrane
dc.subjectcell junction
dc.subjectcontrolled study
dc.subjectcytoplasm
dc.subjectelectron microscopy
dc.subjectembryo
dc.subjectendometrium
dc.subjectestrus
dc.subjectfemale
dc.subjectimmunohistochemistry
dc.subjectimmunoreactivity
dc.subjectmicroscopy
dc.subjectmouse
dc.subjectnidation
dc.subjectnonhuman
dc.subjectuterine tissue
dc.subjectuterus horn
dc.subjectanimal
dc.subjectanimal model
dc.subjectchemistry
dc.subjectdrug effects
dc.subjectepithelium
dc.subjectepithelium cell
dc.subjectmicrovillus
dc.subjectnidation
dc.subjectpregnancy
dc.subjecttransmission electron microscopy
dc.subjectultrastructure
dc.subjectuterus
dc.titleImmunohistochemical and ultrastructural analysis of the effect of omega-3 on embryonic implantation in an experimental mouse model
dc.typeArticle

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