Browsing by Subject "Hep-G2 cell line"

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    Synthesis and cytotoxic activities of organometallic Ru(II) diamine complexes
    (Academic Press Inc., 2020) Kavukcu S.B.; Şahin O.; Seda Vatansever H.; Kurt F.O.; Korkmaz M.; Kendirci R.; Pelit L.; Türkmen H.
    A series of mono and bimetallic ruthenium(II) arene complexes bearing diamine (Ru1-6) were prepared and fully characterized by 1H, 13C, 19F, and 31P NMR spectroscopy and elemental analysis. The crystal structure of the bimetallic complex (Ru5) was determined by X-ray crystallography. Monometallic analogues (Ru1-3) were synthesized to investigate the contributions of ruthenium and the other organic groups (aren, ethylenediamine, butyl) to the activity. The electrochemical behaviors of mono and bimetallic complexes were obtained from the relationship between cyclic voltammetry (CV) and the biological activities of the compounds. The cytotoxic activities of the complexes (Ru1-6) were tested against wide-scale cancer cell lines, namely HeLa, MDA-MB-231, DU-145, LNCaP, Hep-G2, Saos-2, PC-3, and MCF-7, and normal cell lines 3T3-L1 and Vero. Diamine Ru(II) arene complexes have unique biological characteristics and they are promising models for new anticancer drug development. MTT analysis reveals that each synthesized Ru complex showed cytotoxic activity towards the different cancer cells. In particular, three Ru complexes (Ru3, Ru5 and Ru6) showed less toxic effects on the cancer cells than the others. These novel Ru complexes affected both cancer and normal cell lines. As they had a toxic effect on the cells, the dosage applied should be tested before being used for in vivo applications. Cytotoxicity tests have shown that the bimetallic complex Ru6 was effective on all cancer cells. The effect of bimetallic enhancement on cancer cell lines, the systematic variation of the intermetallic distance and the ligand donor properties of the mono and bimetallic complexes were explored based on the cytotoxic activity. The interaction with FS-DNA and the stability/aquation of the complexes (Ru3 and Ru6) were investigated with 1H NMR spectroscopy. The binding modes between the complexes (Ru3 and Ru6) and DNA were investigated via UV–Vis spectroscopy. © 2020 Elsevier Inc.
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    Endoplasmic Reticulum Stress-Induced Apoptotic Effects of Novel 1-Pyrroline (3,4-Dihydro-2H-pyrrole) Derivatives on Breast Cancer Cells
    (John Wiley and Sons Inc, 2022) Atmaca H.; Ilhan S.; Korkmaz E.; Zora M.
    Heterocyclic compounds have emerged as promising and appealing scaffolds for developing effective antitumor agents. Here, the effects of synthesized 24 different 1-pyrroline derivatives (PDs) containing substituted aryl sulfide moiety were investigated on human breast cancer cell lines. The viability of cells was assessed via MTT assay. Reactive oxygen species (ROS) generation was analyzed via fluorescent dye CM-H2DCFDA. Apoptotic cells were determined via flow cytometry. Endoplasmic reticulum (ER) stress-associated protein levels were analyzed via western blot analysis. Four of the PDs (PD-12, -14, -16 and -17) had great cytotoxic selectivity against breast cancer cells. Apoptotic cell death was induced by PDs via the generation of ROS. PDs significantly increased the GRP78, p-PEAK, p-eIF2α, and CHOP protein levels indicating ER stress in breast cancer cells. These results imply that newly synthesized PDs may be potential anticancer agents as they selectively inhibit breast cancer cells. © 2022 Wiley-VHCA AG, Zurich, Switzerland.
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    Anti-Tumorigenic Effect of Resveratrol in HepG2 cells Controlled with Cytochrome-c Dependent Cell Death
    (Mattioli 1885, 2022) Kıyak N.; Becer E.; Vatansever H.S.; Kükner A.
    Resveratrol is a phytochemical that is regarded as a potential anticancer agent in liver cancer prevention. It had also been shown that resveratrol has role in the prevention of cancer and anti-cancer properties. In this study, we aimed to investigate the effects of resveratrol on cell viability, apoptosis, cellular proliferation, JAK/STAT pathway and epithelial-mesenchymal transition (EMT) in human hepatocellular carcinoma cell (HepG2) line. Cell growth and cytotoxicity were evaluated with MTT assay with different concentrations (5, 10, 25, 50, 100 μM) of resveratrol in HepG2 cells. The distribution of FasL, cyt-c, caspase-3, Ki-67, ACTA2, CD133, JAK2, N-cadherin, vimentin and STAT3 in HepG2 cells were analyzed using indirect immunoperoxidase technique. The effective dose and incubation time for inhibition of cell growth in HepG2 cells was determined as 100 μM for 48 hours. Decreased Ki-67 immunoreactivity following resveratrol application was significant in HepG2 cells. Increased cytc-c, STAT3, vimentin, N-cadherin and CD133 immunoreactivities were significant between resveratrol applicated HepG2 cells and control group. According to our results, resveratrol induced mitochondrial-dependent cell death and suppressed proliferation in HepG2 cells. On the other hand, our results showed that resveratrol stimulated cellular self-protection responses through activation of EMT and STAT3 protein expression in HepG2 cells. © 2022 Mattioli 1885. All rights reserved.
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    Assessment of in-vitro cytotoxicity and in-ovo virucidal antiviral efficacy of various plant extracts and bioactive molecules
    (Veteriner Fakultesi Dergisi, 2024) Çöven F.O.; Gür S.; Uyar E.; Alsakini K.A.M.H.; Karabey F.; Çöven F.; Çaliş İ.; Nalbantsoy A.
    The viral diseases that occurred in recent years have increased the interest in non-toxic to healthy cells and naturally isolated agents to struggle with these diseases. The key intention of this research is to examine both antiviral potentials against the Infectious Bronchitis model virus (IBV) and cytotoxic activities on determined cell lines of different active ingredients and medical herbs extracts for developing new antiviral agents or drugs towards SARS-CoV-2. The antiviral potency of the samples against IBV was determined as in ovo virucidal antiviral activity in specific pathogen-free (SPF) embryonated chicken eggs (ECEs). To detect antiviral activity, the haemagglutination test was performed after 48 h of incubation for all samples. The cytotoxic activity of the samples was identified on HepG2, Caco-2, HeLa, HEK293, PANC-1, PC-3, A549, MDA-MB-231, and CCD-34Lu cell lines by the MTT protocol. Hypericum perforatum extract was found to have a dominant role in cytotoxicity and antiviral activity. In addition, while nobiletin and Sambucus nigra do not exhibit cytotoxic activity on cells, they play a significant role in antiviral activity. As a consequence of our investigation, the cytotoxic and antiviral properties of Laurus nobilis, H. perforatum, and S. nigra extracts were found remarkable and the potential of these extracts was demonstrated. © 2024, Veteriner Fakultesi Dergisi. All rights reserved.
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    A newly synthesized thiosemicarbazide derivative trigger apoptosis rather than necroptosis on HEPG2 cell line
    (John Wiley and Sons Inc, 2024) Başoğlu-Ünal F.; Becer E.; Ensarioğlu H.K.; -Güzeldemirci N.U.; Kuran E.D.; Vatansever H.S.
    Thiosemicarbazide derivatives have been the focus of scientists owing to their broad biological activities such as anticancer, antimicrobial, and anti-inflammatory. Herein, we designed and synthesized a new thiosemicarbazide derivative (TS-1) and evaluated its antiproliferative potential against the human hepatocellular carcinoma cell line (HEPG2) and human umbilical vein endothelial cell line (ECV-304). Also, it was aimed to investigate the necroptotic and apoptotic cell death effects of TS-1 in HEPG2 cells, and these effects were supported by molecular docking. The new synthesized compound structure was characterized using various spectroscopic methods such as FT-IR, 1H-NMR, 13C-NMR, and elemental analysis. The cytotoxic activity of the tested compound was measured by the MTT assay. Apoptotic and necroptotic properties of the TS-1 were evaluated by indirect immunoperoxidase method using antibodies against Ki-67, Bax, Bcl-2, caspase-3, caspase-8, caspase-9, RIP3, and RIPK1. Apoptotic and necroptotic effects of TS-1 were supported by molecular docking. Compound TS-1 was synthesized as a pure compound with a high yield. The effective value of TS-1 was 10 μM in HEPG2 cells. TS-1 did not show any cytotoxic effect on ECV-304. Caspase-3 and RIPK1 immunoreactivities were significantly increased in HEPG2 cells after being treated with TS-1. As the results of the molecular docking studies, the molecular docking showed that the TS-1 exhibits H-bond interaction with various significant amino acid residues in the active site of both RIPK1. It could be concluded that TS-1 could be a promising novel therapeutic agent by inducing apoptosis rather than necroptosis in HEPG2 cells. © 2023 John Wiley & Sons Ltd.