Endoplasmic Reticulum Stress-Induced Apoptotic Effects of Novel 1-Pyrroline (3,4-Dihydro-2H-pyrrole) Derivatives on Breast Cancer Cells
No Thumbnail Available
Date
2022
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Heterocyclic compounds have emerged as promising and appealing scaffolds for developing effective antitumor agents. Here, the effects of synthesized 24 different 1-pyrroline derivatives (PDs) containing substituted aryl sulfide moiety were investigated on human breast cancer cell lines. The viability of cells was assessed via MTT assay. Reactive oxygen species (ROS) generation was analyzed via fluorescent dye CM-H2DCFDA. Apoptotic cells were determined via flow cytometry. Endoplasmic reticulum (ER) stress-associated protein levels were analyzed via western blot analysis. Four of the PDs (PD-12, -14, -16 and -17) had great cytotoxic selectivity against breast cancer cells. Apoptotic cell death was induced by PDs via the generation of ROS. PDs significantly increased the GRP78, p-PEAK, p-eIF2α, and CHOP protein levels indicating ER stress in breast cancer cells. These results imply that newly synthesized PDs may be potential anticancer agents as they selectively inhibit breast cancer cells. © 2022 Wiley-VHCA AG, Zurich, Switzerland.
Description
Keywords
1 pyrroline(3,4 dihydro 2h pyrrole)derivative , antineoplastic agent , cisplatin , endoplasmic reticulum chaperone BiP , fluorescent dye , fluorouracil , growth arrest and DNA damage inducible protein 153 , initiation factor 2alpha , protein Bax , pyrrole derivative , reactive oxygen metabolite , unclassified drug , antiapoptotic activity , Article , breast cancer cell line , cancer chemotherapy , cancer immunotherapy , cell proliferation , cell viability , controlled study , cytotoxicity , endoplasmic reticulum stress , female , flow cytometry , folate metabolism , Hep-G2 cell line , human , human cell , IC50 , MCF-7 cell line , MTT assay , protein expression level , quality of life , Western blotting