Browsing by Subject "cryoprotective agent"

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    [Cryopreservation of plasmodia with malaria models and establishment of a cryobank].; [Sitma modeli etkenleri ile kriyoprezervasyon çalişmalari ve kriyobanka oluşturulmasi.]
    (2010) Özbilgin A.; Östan I.; Tabak T.; Aşar K.
    Cryopreservation is simply a method of keeping living cells frozen with the chance of regaining cellular viability, functions and antigenic structures whenever required, after heating. In the present study, dimethyl sulphoxide (DMSO) was mixed with the red blood cells having 20% of parasitemia obtained from the mice infected with Plasmodium yoelii and Plasmodium berghei at a final concentration of 15%. For cryopreservation: both test tubes containing each Plasmodium species were kept 10 minutes at room temperature, 30 minutes at +4°C, 90 minutes at -20°C and finally at -80°C. Some were left at this temperature, while some were transferred into the liquid nitrogen tank at -196°C after being left at -80°C for three hours. Our observations and assessments demonstrated that both P. yoelii and P. berghei might keep their viability and virulence at -80°C and -196°C between the first and the sixth months of cryopreservation. It can be concluded that the cryopreservation of P. yoelii and P. berghei at -80°C and -196°C are successful, indicating the advantage of the establishment of parasite cryobanks in research laboratories.
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    Cryopreservation of Leishmania Species in Manisa Province
    (2017) Çavuş İ.; Ocak F.; Kaya T.; Özbilgin A.
    OBJECTIVE: It was aimed to assess the success of the cryopreservation process which is carried out in order to preserve the genetic material and the virulence of the Leishmania species that are an important health problem in our region.; METHODS: Leishmania tropica, L. infantum, L. major, and L. donovani strains in Novy-MacNeal-Nicolle (NNN) medium in MCBU were used. Promastigotes cultured in the NNN medium were transferred to RPMI 1640 medium; promastigotes in the logarithmic phase were washed three times with PBS, and 15% dimethylsulfoxide (DMSO) was added. Leishmania species were transferred to 12 separate tubes. The tubes were stored at -86°C for one night by placing them in Coolcell boxes. The tubes were transferred into a liquid nitrogen tank. One cryotube per Leishmania strain is thawed monthly and cultured in NNN medium.; RESULTS: For the duration of study it was observed that each Leishmania isolate preserved 60-65% of their viability and entered the logarithmic phase on the 7th day following the inoculation in the NNN medium. Abnormalities in the structures and movements of the promastigotes were not observed in microscopic examinations.; CONCLUSION: The following conclusions were made: cryopreservation is important for studies planned related to leishmaniasis and cryopreservation with DMSO is successful.