Expression profiles of histone modification genes in gastric cancer progression
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Date
2018
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Abstract
Gastric cancer (GC) development can be attributed to several risk factors including atrophic gastritis (AG), intestinal metaplasia (IM), and the presence of Helicobacter pylori (HP). Also, histone modification is an epigenetic mechanism that plays a pivotal role in GC carcinogenesis. In this preliminary study, we aimed to describe the expression profiles of histone modification in the AG, IM, and GC patient groups. A total of 80 patients with AG (n = 27), IM (n = 25), and GC (n = 28) with an additional 20 control subjects were included in the study. Expression profiles of three histone phosphorylation genes (PAK1, NEK6, and AURKA) and five histone deacetylation genes (HDACs 1, 2, 3, 5, and 7) were examined based on the results of Real Time qPCR method. It was observed that AURKA and HDAC2 genes were significantly overexpressed in all groups compared to the control (P < 0.05). In GC patients, overexpression of HDAC2 gene was detected in the absence of metastasis, and overexpression of AURKA, HDAC2, and NEK6 genes was detected in the presence of metastasis. When cancer involvements were compared, significant overexpression of the HDAC2 gene was noted in overall and corpus involvements (P < 0.05). In addition, overexpression of AURKA, NEK6, HDAC1, and HDAC2 genes and underexpression of HDAC5 gene were detected in the antrum involvement (P < 0.05). In conclusion, decreased expression of HDAC5 in GC is reported for the first time in this study, while supporting the existing literature in AURKA, NEK6, HDAC1, and HDAC2 up regulations during GC development. © 2018, Springer Nature B.V.
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Adult , Aged , Aurora Kinase A , Disease Progression , Female , Gastric Mucosa , Gastritis, Atrophic , Genetic Variation , Helicobacter Infections , Helicobacter pylori , Histone Code , Histone Deacetylases , Histones , Humans , Intestines , Male , Metaplasia , Middle Aged , NIMA-Related Kinases , p21-Activated Kinases , Preliminary Data , Risk Factors , Stomach , Stomach Neoplasms , Transcriptome , aurora A kinase , histone , histone deacetylase 1 , histone deacetylase 2 , histone deacetylase 3 , histone deacetylase 5 , p21 activated kinase 1 , AURKA protein, human , HDAC5 protein, human , histone , histone deacetylase , NEK6 protein, human , NIMA related kinase , p21 activated kinase , PAK1 protein, human , transcriptome , adult , Article , atrophic gastritis , AURKA gene , cancer growth , cancer patient , controlled study , female , gene , gene expression , gene expression profiling , gene overexpression , HDAC1 gene , HDAC2 gene , HDAC3 gene , HDAC5 gene , HDAC7 gene , Helicobacter pylori , histone acetylation , histone modification , human , intestine metaplasia , major clinical study , male , metastasis , NEK6 gene , PAK1 gene , pathogenesis , stomach antrum , stomach cancer , upregulation , aged , disease exacerbation , genetic variation , genetics , Helicobacter infection , histone code , intestine , metaplasia , middle aged , pathology , preliminary data , risk factor , stomach , stomach mucosa , stomach tumor