Regulation of apoptosis-related molecules by synergistic combination of all-trans retinoic acid and zoledronic acid in hormone-refractory prostate cancer cell lines

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dc.contributor.authorKarabulut B.
dc.contributor.authorKaraca B.
dc.contributor.authorAtmaca H.
dc.contributor.authorKisim A.
dc.contributor.authorUzunoglu S.
dc.contributor.authorSezgin C.
dc.contributor.authorUslu R.
dc.date.accessioned2024-07-22T08:20:31Z
dc.date.available2024-07-22T08:20:31Z
dc.date.issued2011
dc.description.abstractWe report that all-trans retinoic acid (ATRA) in combination with zoledronic acid has strong synergistic cytotoxic and apoptotic effects against human hormone- and drug-refractory prostate cancer cells, PC-3 and DU-145, in a time- and dose-dependent manner. We further investigated the effect of the combination treatment on the apoptotic process by both oligoarray and protein array analysis in DU-145 cells, in which the drug combination shows much more strong synergistic effects, as compared to PC-3 cells. Moreover, we have also performed real time-PCR array analysis to validate oligoarray results. We demonstrated that the combination of ATRA and zoledronic acid is a strong inducer of apoptotic related cell death in human androgen-and drug refractory prostate cancer cells DU-145, at either transcriptional or translational levels. While expression of proapoptotic genes such as tumor necrosis factor receptor superfamily (TNFRSF), Bad, Bax, Fas, FADD are induced with the exposure of the combination, expression of antiapoptotic genes or proteins such as members of inhibitor apoptosis family (IAPs), MCL-1, LTBR, p53 and bcl-2 are reduced. Because this novel combination treatment has fewer side effects than is generally the case with conventional cytotoxic agents, this regimen may be a good option for treatment of elderly prostate cancer patients. © 2010 Springer Science+Business Media B.V.
dc.identifier.DOI-ID10.1007/s11033-010-0102-6
dc.identifier.issn15734978
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18177
dc.language.isoEnglish
dc.subjectAntineoplastic Agents
dc.subjectApoptosis
dc.subjectApoptosis Regulatory Proteins
dc.subjectCaspase 3
dc.subjectCaspase 7
dc.subjectCell Death
dc.subjectCell Line, Tumor
dc.subjectCell Proliferation
dc.subjectCell Survival
dc.subjectDiphosphonates
dc.subjectDNA Fragmentation
dc.subjectDose-Response Relationship, Drug
dc.subjectDrug Synergism
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectHormones
dc.subjectHumans
dc.subjectImidazoles
dc.subjectMale
dc.subjectProstatic Neoplasms
dc.subjectReceptors, Tumor Necrosis Factor
dc.subjectReproducibility of Results
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA, Messenger
dc.subjectTime Factors
dc.subjectTretinoin
dc.subjectIntracisternal A-particles
dc.subjectcaspase 3
dc.subjectcaspase 7
dc.subjectcytochrome c
dc.subjectFas associated death domain protein
dc.subjectFas ligand
dc.subjectheme oxygenase 2
dc.subjectlivin
dc.subjectlymphotoxin beta receptor
dc.subjectprotein BAD
dc.subjectprotein Bax
dc.subjectprotein bcl 2
dc.subjectprotein mcl 1
dc.subjectprotein p53
dc.subjectretinoic acid
dc.subjectsecond mitochondrial activator of caspase
dc.subjectsurvivin
dc.subjecttumor necrosis factor receptor
dc.subjecttumor necrosis factor receptor superfamily
dc.subjectunclassified drug
dc.subjectX linked inhibitor of apoptosis
dc.subjectzoledronic acid
dc.subjectapoptosis
dc.subjectarticle
dc.subjectcancer cell culture
dc.subjectcancer inhibition
dc.subjectcell proliferation
dc.subjectcell viability
dc.subjectcontrolled study
dc.subjectcytotoxicity
dc.subjectDNA fragmentation
dc.subjectdose response
dc.subjectdrug potentiation
dc.subjectenzyme activation
dc.subjectenzyme activity
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmale
dc.subjectprostate cancer
dc.subjectprotein expression
dc.subjectreal time polymerase chain reaction
dc.titleRegulation of apoptosis-related molecules by synergistic combination of all-trans retinoic acid and zoledronic acid in hormone-refractory prostate cancer cell lines
dc.typeArticle

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