Photostimulation of osteogenic differentiation on silk scaffolds by plasma arc light source

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dc.contributor.authorÇakmak, AS
dc.contributor.authorÇakmak, S
dc.contributor.authorVatansever, HS
dc.contributor.authorGümüsderelioglu, M
dc.date.accessioned2024-07-18T11:52:02Z
dc.date.available2024-07-18T11:52:02Z
dc.description.abstractLow-level laser therapy (LLLT) has been used for more than 30 years to heal wounds. In recent years, LLLT or photostimulation has been indicated as an effective tool for regenerative and dental medicine by using monochromatic light. The aim of this study is to indicate the usability of plasma arc light source for bone regeneration. This is why we used polychromatic light source providing effective wavelengths in the range of 590-1500 nm for cellular response and investigated photostimulation effects on osteogenic differentiation of human mesenchymal stem cells (hMSCs) seeded on 3D silk scaffolds. Cellular responses were examined by using cell culture methods in terms of proliferation, differentiation, and morphological analyses. The results showed that photostimulation with a polychromatic light source (applied for 5 min from the 3rd day after seeding up to the 28th day in 2-day intervals with 92-mW/cm(2) power from 10-cm distance to the cells) enhanced osteogenic differentiation of hMSCs according to higher alkaline phosphatase (ALP) activity, collagen and calcium content, osteogenic gene expressions, and matrix mineralization. In conclusion, we suggest that the plasma arc light source that was used here has a great potential for bone regeneration.
dc.identifier.issn0268-8921
dc.identifier.other1435-604X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/5323
dc.language.isoEnglish
dc.publisherSPRINGER LONDON LTD
dc.subjectLEVEL LASER THERAPY
dc.subjectMESENCHYMAL STEM-CELLS
dc.subjectBONE-MARROW-CELLS
dc.subjectPROMOTES PROLIFERATION
dc.subjectIRRADIATION PROMOTES
dc.subjectGASTROCNEMIUS-MUSCLE
dc.subjectIN-VITRO
dc.subjectOSTEOBLASTS
dc.subjectREGENERATION
dc.subjectLLLT
dc.titlePhotostimulation of osteogenic differentiation on silk scaffolds by plasma arc light source
dc.typeArticle

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