Isolation and Purification of Two Metabolites (KGG32-A & KGG32-B) from a Soil Bacterium, Streptomyces sp., KGG32
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This research is concerned with the isolation, purification and partial characterization of two metabolites from culture filtrate of Streptomyces sp. KGG32, by solvent extraction, silica gel chromatography, UV and FT-IR spectroscopic analyses. This strain is a highly stable antimicrobial metabolite producer against different microorganisms such as S. aureus, B. cereus, B. subtilis, Kocuria rhizophila, E. coli, Enterococcus faecalis and C. albicans under in-vitro growth conditions. In order to obtain crude extract, ten liters of the fermentation broth was extracted with different solvents and concentrated until dryness. The crude extract was subjected to silica gel column chromatography and the fractions showing the highest antimicrobial activity were pooled and determined by TLC for purity. Extraction of fermentation broth, various separation and purification steps led to isolation of two pure active compounds having a retention factor of 0.22 (methanol: water, 3:1, v/v) and 0.64 (ethanol: water, 3:1, v/v), respectively. MIC values of the isolated compounds were found between 5.209-25.0 mu g/mL. (C) 2011 Friends Science Publishers