Development of a Radiolabeled Folate-Mediated Drug Delivery System for Effective Delivery of Docetaxel

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dc.contributor.authorÇetin, O
dc.contributor.authorGüngör, B
dc.contributor.authorIçhdef, C
dc.contributor.authorParlak, Y
dc.contributor.authorBilgin, ES
dc.contributor.authorÜstün, F
dc.contributor.authorAltun, GD
dc.contributor.authorBaspinar, Y
dc.contributor.authorTeksöz, S
dc.date.accessioned2024-07-18T12:05:12Z
dc.date.available2024-07-18T12:05:12Z
dc.description.abstractMany preclinical studies are carried out with the aimof developingnew formulations for the effective delivery of taxane class drugs,one of the most important anticancer drugs used clinically today.In this study, a radiolabeled folate-mediated solid lipid magneticnanoparticle (SLMNP) system was developed by loading superparamagneticiron oxide nanoparticles (MNP) and docetaxel (DTX) into the solidlipid nanoparticles as a drug delivery system that will function bothin cancer treatment and diagnosis. For this purpose, first, SLMNPwas synthesized by the hot homogenization method, and the surfaceof the particles was modified with a folate derivative to carry theparticles to tissues with folate receptors. The synthesized magneticsolid lipid nanoparticles were loaded with DTX, and then radiolabelingwas carried out with technetium-99 m (Tc-99m-DTX-SLMNP).Structural characteristics of these nanoparticles were determinedby characterization methods. According to the TEM images of MNPs,SLN, and SLMNPs, MNPs were observed between 25and 35 nm, SLNs between400 and 500 nm, and SLMNPs between 350 and 450 nm. The drug entrapmentefficiency of SLMNPs loaded with DTX was found to be 19%, and thepercentage efficiency of radiolabeling was found to be 98.0 & PLUSMN;2.0%. The biological behavior of this radiolabeled system was investigated in vitro and in vivo. Folate receptor-positiveSKOV-3 and folate receptor-negative A549 cancer cell lines were studied.The IC50 values of DTX-SLMNP in SKOV-3 and A549 cells were50.21 and 172.27 & mu;M at 48 h, respectively. Gamma camera imagingstudies of Tc-99m-DTX-SLMNP and magnetically applied Tc-99m-DTX-SLMNP compounds were performed on tumor-bearing CD-1nude mice. The uptake in the folate receptor-positive tumor regionwas higher than that in the folate receptor negative tumor region.We proposed that the drug delivery system we prepared in this studybe evaluated for preclinical studies of new drug carrier formulationsof the taxane class of anticancer drugs.
dc.identifier.issn2470-1343
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/9595
dc.language.isoEnglish
dc.publisherAMER CHEMICAL SOC
dc.subjectSOLID LIPID NANOPARTICLES
dc.titleDevelopment of a Radiolabeled Folate-Mediated Drug Delivery System for Effective Delivery of Docetaxel
dc.typeArticle

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