Anti-cancer efficiency of natural killer cells differentiated from human adipose tissue-derived mesenchymal stem cells and transfected with miRNA150
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Date
2017
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Abstract
Aim: The aim of this study is to investigate the effects of miR150 transfection on NK-like cells differentiated from adipose tissue derived mesenchymal stem cells (AD-MSCs). Methods: NK-like cells were differentiated from AD-MSCs and activated by miR150 transfection. Transfected/non-Transfected NK-like cells were characterized by immunohistochemical and RTPCR analyzes. Apoptotic efficiency of the transfected/non-Transfected NK-like cells on pancreatic cancer cells PANC1 were determined by TUNEL and RT-PCR. Results: In miR150-Transfected cells, the increased expression of NK cell-specific genes such as GKMB, KIR2DL2, CD16, CD56, NKG2D, NKp46 and increased immunoreactivity of NK cell-specific surface marker CD314 (NKG2D) were evident. TUNEL assays showed that NK-like cells with/without transfection induced apoptosis in PANC1 cells in the same manner. The decrease in oncogene expression and the increase in the tumor suppressor gene expression in PANC1 cells upon co-culture with NK-like cells differentiated from AD-MSCs were more prominent following miRNA150 transfection. Conclusion: It was shown in vitro that NK-like cells could be obtained by differentiation from AD-MSCs and their efficiency could be increased via miR150 transfection. The results are encouraging for further clinical studies in improvement of immunotherapeutic approaches for cancer therapy. Copyright © Experimental Oncology, 2017.
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Adipose Tissue , Apoptosis , Biomarkers , Cell Differentiation , Cell Line, Tumor , Cells, Cultured , Coculture Techniques , Cytotoxicity, Immunologic , Gene Expression , Humans , Killer Cells, Natural , Mesenchymal Stromal Cells , MicroRNAs , Neoplasms , Transfection , microRNA , microRNA 150 , natural killer cell receptor NKG2D , unclassified drug , biological marker , microRNA , MIRN150 microRNA, human , adipose tissue cell , adipose tissue derived mesenchymal stem cell , antineoplastic activity , apoptosis , Article , CD16 gene , CD56 gene , cell differentiation , coculture , controlled study , gene expression regulation , genetic transfection , GKMB gene , human , human cell , immunohistochemistry , immunoreactivity , KIR2DL2 gene , mesenchymal stem cell , natural killer cell , NK cell culture , NKG2D gene , NKp46 gene , oncogene , PANC-1 cell line , reverse transcription polymerase chain reaction , stem cell culture , tumor suppressor gene , TUNEL assay , upregulation , adipose tissue , cell culture , cell differentiation , cytology , cytotoxicity , gene expression , genetics , immunology , mesenchymal stroma cell , metabolism , natural killer cell , neoplasm , pathology , tumor cell line