Anti-cancer efficiency of natural killer cells differentiated from human adipose tissue-derived mesenchymal stem cells and transfected with miRNA150

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dc.contributor.authorKarlitepe A.
dc.contributor.authorKabadayi H.
dc.contributor.authorVatansever S.
dc.contributor.authorGurdal M.
dc.contributor.authorGunduz C.
dc.contributor.authorErcan G.
dc.date.accessioned2024-07-22T08:10:44Z
dc.date.available2024-07-22T08:10:44Z
dc.date.issued2017
dc.description.abstractAim: The aim of this study is to investigate the effects of miR150 transfection on NK-like cells differentiated from adipose tissue derived mesenchymal stem cells (AD-MSCs). Methods: NK-like cells were differentiated from AD-MSCs and activated by miR150 transfection. Transfected/non-Transfected NK-like cells were characterized by immunohistochemical and RTPCR analyzes. Apoptotic efficiency of the transfected/non-Transfected NK-like cells on pancreatic cancer cells PANC1 were determined by TUNEL and RT-PCR. Results: In miR150-Transfected cells, the increased expression of NK cell-specific genes such as GKMB, KIR2DL2, CD16, CD56, NKG2D, NKp46 and increased immunoreactivity of NK cell-specific surface marker CD314 (NKG2D) were evident. TUNEL assays showed that NK-like cells with/without transfection induced apoptosis in PANC1 cells in the same manner. The decrease in oncogene expression and the increase in the tumor suppressor gene expression in PANC1 cells upon co-culture with NK-like cells differentiated from AD-MSCs were more prominent following miRNA150 transfection. Conclusion: It was shown in vitro that NK-like cells could be obtained by differentiation from AD-MSCs and their efficiency could be increased via miR150 transfection. The results are encouraging for further clinical studies in improvement of immunotherapeutic approaches for cancer therapy. Copyright © Experimental Oncology, 2017.
dc.identifier.DOI-ID10.31768/2312-8852.2017.39(3):212-218
dc.identifier.issn18129269
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15370
dc.language.isoEnglish
dc.publisherMorion LLC
dc.rightsAll Open Access; Green Open Access
dc.subjectAdipose Tissue
dc.subjectApoptosis
dc.subjectBiomarkers
dc.subjectCell Differentiation
dc.subjectCell Line, Tumor
dc.subjectCells, Cultured
dc.subjectCoculture Techniques
dc.subjectCytotoxicity, Immunologic
dc.subjectGene Expression
dc.subjectHumans
dc.subjectKiller Cells, Natural
dc.subjectMesenchymal Stromal Cells
dc.subjectMicroRNAs
dc.subjectNeoplasms
dc.subjectTransfection
dc.subjectmicroRNA
dc.subjectmicroRNA 150
dc.subjectnatural killer cell receptor NKG2D
dc.subjectunclassified drug
dc.subjectbiological marker
dc.subjectmicroRNA
dc.subjectMIRN150 microRNA, human
dc.subjectadipose tissue cell
dc.subjectadipose tissue derived mesenchymal stem cell
dc.subjectantineoplastic activity
dc.subjectapoptosis
dc.subjectArticle
dc.subjectCD16 gene
dc.subjectCD56 gene
dc.subjectcell differentiation
dc.subjectcoculture
dc.subjectcontrolled study
dc.subjectgene expression regulation
dc.subjectgenetic transfection
dc.subjectGKMB gene
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunohistochemistry
dc.subjectimmunoreactivity
dc.subjectKIR2DL2 gene
dc.subjectmesenchymal stem cell
dc.subjectnatural killer cell
dc.subjectNK cell culture
dc.subjectNKG2D gene
dc.subjectNKp46 gene
dc.subjectoncogene
dc.subjectPANC-1 cell line
dc.subjectreverse transcription polymerase chain reaction
dc.subjectstem cell culture
dc.subjecttumor suppressor gene
dc.subjectTUNEL assay
dc.subjectupregulation
dc.subjectadipose tissue
dc.subjectcell culture
dc.subjectcell differentiation
dc.subjectcytology
dc.subjectcytotoxicity
dc.subjectgene expression
dc.subjectgenetics
dc.subjectimmunology
dc.subjectmesenchymal stroma cell
dc.subjectmetabolism
dc.subjectnatural killer cell
dc.subjectneoplasm
dc.subjectpathology
dc.subjecttumor cell line
dc.titleAnti-cancer efficiency of natural killer cells differentiated from human adipose tissue-derived mesenchymal stem cells and transfected with miRNA150
dc.typeArticle

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