Neuroprotective effects of catechins in an experimental Parkinson's disease model and SK-N-AS cells: evaluation of cell viability, anti-inflammatory and anti-apoptotic effects

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dc.contributor.authorÖzduran, G
dc.contributor.authorBecer, E
dc.contributor.authorVatansever, HS
dc.contributor.authorYücecan, S
dc.date.accessioned2024-07-18T12:02:41Z
dc.date.available2024-07-18T12:02:41Z
dc.description.abstractObjectives The aim of the study was to establish an in vitro Parkinson's disease (PD) model and to investigate the cell viability, anti-inflammatory, anti-apoptotic and neuroprotective effects of catechin and EGCG in SK-N-AS and in vitro PD model cells. Method SK-N-AS human neuroblastoma cells were used. To develop an in vitro PD model, SK-N-AS cells were exposed to 6-hydroxydopamine. Model control was performed after ELISA analysis of dopamine and alpha-synuclein levels in the culture medium. Catechin and EGCG were administered to SK-N-AS and in vitro PD model cells. Cell viability was measured using MTT assay and trypan blue staining. Anti-inflammatory and anti-apoptotic activities of catechin and EGCG were investigated by indirect immunocytochemistry using anti-TNF-alpha, anti-IL-1 beta and anti-caspase-3. Results After 24 hours of 6-hydroxydopamine administration at 50 mu M, higher alpha lfa-synuclein and lower dopamine levels were found in PD model than SK-N-AS cells. Cell viability was similar between SK-N-AS and in vitro PD model cells. Treatment with both bioactive components increased cell viability of in vitro PD model cells. Caspase-3 immunoreactivity was significantly reduced in SK-N-AS and PD model cells after EGCG administration, while it was decreased only in PD model cells after catechin administration. IL-1 beta staining intensity weakened after catechin administration in PD model cells, after EGCG administration in SK-N-AS cells. TNF-alpha staining intensity was similar in both cells. Conclusion Catechin and EGCG increased cell viability in PD model neuron cells. Both components showed anti-apoptotic and anti-inflammatory effects. Catechin may be more effective in preventing damage to neurons PD.
dc.identifier.issn0161-6412
dc.identifier.other1743-1328
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/8604
dc.language.isoEnglish
dc.publisherTAYLOR & FRANCIS LTD
dc.subjectALPHA-SYNUCLEIN AGGREGATION
dc.subjectNEURODEGENERATIVE DISEASES
dc.subjectEPIGALLOCATECHIN GALLATE
dc.subject(-)-EPIGALLOCATECHIN-3-GALLATE
dc.subjectDEATH
dc.titleNeuroprotective effects of catechins in an experimental Parkinson's disease model and SK-N-AS cells: evaluation of cell viability, anti-inflammatory and anti-apoptotic effects
dc.typeArticle

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