Evaluation of the performance of maldi-tof ms and dna sequence analysis in the identification of mycobacteria species

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dc.contributor.authorAkyar I.
dc.contributor.authorÇavuşoğlu C.
dc.contributor.authorAyaş M.
dc.contributor.authorSürücüoğlu S.
dc.contributor.authorİlki A.
dc.contributor.authorKaya D.E.
dc.contributor.authorBeşli Y.
dc.date.accessioned2025-04-10T11:08:09Z
dc.date.available2025-04-10T11:08:09Z
dc.date.issued2018
dc.description.abstractBackground/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permits early, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to standard sequence analysis. Materials and methods: A total of 155 Mycobacterium clinical and external quality control isolates, comprising nontuberculous mycobacteria (NTM) (n = 95) and the Mycobacterium tuberculosis complex (MTC) (n = 60), were included in the study. Results: Identification by MBT ML4.0 was correctly performed in 100% of MTC and in 91% of NTM isolates. All of the MTC isolates were correctly differentiated from NTM isolates. Conclusion: Based on our results, MBT ML4.0 may be used reliably to identify both NTM and MTC. © TÜBİTAK.
dc.identifier.DOI-ID10.3906/sag-1801-198
dc.identifier.urihttp://hdl.handle.net/20.500.14701/47826
dc.publisherTurkiye Klinikleri Journal of Medical Sciences
dc.titleEvaluation of the performance of maldi-tof ms and dna sequence analysis in the identification of mycobacteria species
dc.typeArticle

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