TRP Channels in Tension-Type Headache: A Pilot Study; [Gerilim Tipi Baş Ağrısında TRP Kanalları: Pilot Çalışma]
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Date
2021
Authors
Gemici Y.İ.
Taşcı İ.
Durmuş K.
Koç A.
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Abstract
Tension-type headache (TTH) affects many individuals worldwide. Although the exact pathogenesis of TTH remains unclear, central, and peripheral mechanisms are considered to play a role in TTH 1. This pilot study aimed to investigate the role of transient receptor potential (TRP) channels in the development or chronic inflammation in TTH and to discuss the findings in the light of literature. This pilot study included a patient group comprising three patients with episodic TTH and three patients with chronic TTH (CTTH) aged 18-40 years with no comorbidities and a control group of three patients with no headache. Peripheral blood samples were obtained from all the participants, and both RNA and cDNA were isolated on the same day. The mRNA levels of pain-related TRP channels [TRPA1, TRP vanilloid-1 (TRPV1), TRPV3, TRPV4, TRPM3, and TRPM8] were measured by reverse transcriptase (RT)-quantititave polymerase chain reaction method and were normalized with the levels of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) transcript. Results were analyzed using statistical methods. All three groups were comparable with regard to demographic characteristics. No significant difference was found among the groups with regard to the mRNA levels of the TRP channels normalized by GAPDH, whereas the TRPM8 expression levels were not significantly lower in the CTTH group than in other groups (p=0.066). This study revealed that TRPM8 is likely to have a role in the pathogenesis of TTH, and this role of TRPM8 may be investigated by further studies. © 2021 by Turkish Neurological Society Turkish Journal of Neurology published by Galenos Publishing House.
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Keywords
complementary DNA, glyceraldehyde 3 phosphate dehydrogenase, RNA directed DNA polymerase, transient receptor potential channel, transient receptor potential channel A1, transient receptor potential channel M8, vanilloid receptor 1, vanilloid receptor 3, vanilloid receptor 4, adult, Article, chronic tension headache, clinical article, clinical feature, comorbidity, DNA methylation, female, gene amplification, gene expression, genetic transcription, genotype, human, male, mRNA expression level, nausea and vomiting, photophobia, pilot study, real time polymerase chain reaction, RNA extraction, tension headache