Quercetin-mediated apoptosis and cellular senescence in human colon cancer
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Date
2020
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Abstract
Background: Quercetin is a flavonol from the flavonoid group of polyphenols, which positively affects human health due to its anti-cancer, anti-inflammatory, anti-microbial and cardioprotective effects. The effects of phenolic compounds, including quercetin, on programmed cell death and cellular senescence, have been the subject of research in recent years. Objective: In this study, we aimed to investigate the effects of quercetin on cell viability, apoptosis and cellular senescence in primary (Colo-320) and metastatic (Colo-741) colon adenocarcinoma cell lines. Methods: Cytotoxicity was analyzed via MTT assay in Colo-320 and Colo-741 cell lines. After quercetin treatment, cell ularsenescence and apoptosis were evaluated by TUNEL staining, X-Gal staining and indirect peroxidase tech-nique for immunocytochemical analysis of related proteins such as Bax, Bcl-2, caspase-3, Hsp27, Lamin B1, p16, cyclin B1. Results: The effective dose for inhibition of cell growth in both cell lines was determined to be 25µg/ml quercetin for 48 hours. Increased Baximmunoreactivityfollowingquercetin treatment was significant in both Colo-320 and Colo-741 cell lines, but decreased Bcl-2 immunoreactivitywas significant only in theColo-320 primary cell line. In addition, after quercetin administration, the number of TUNEL positive cells and, immunoreactivities for p16, Lamin B1 and cyclin B1 in both Colo-320 and Colo-741 cells increased. Conclusion: Our results suggest that quercetin may only induce apoptosis in primary colon cancer cells. Further-more, quercetin also triggered senescence in colon cancer cells, but some cells remained alive, suggesting that colon cancer cells might have escaped from senescence. © 2020 Bentham Science Publishers.
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Antineoplastic Agents , Apoptosis , Cell Proliferation , Cell Survival , Cells, Cultured , Cellular Senescence , Colonic Neoplasms , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Humans , Molecular Structure , Quercetin , Structure-Activity Relationship , caspase 3 , cyclin B1 , diaminobenzidine , distilled water , edetic acid , ferric ferrocyanide , glutamine , heat shock protein 27 , hematoxylin , lamin B , Lamin B1 , magnesium chloride , paraformaldehyde , penicillin derivative , peroxidase , phosphate buffered saline , protein Bax , protein bcl 2 , protein p16 , quercetin , sodium chloride , streptavidin , streptomycin , tetrazolium , triton x 100 , trypsin , unclassified drug , antineoplastic agent , quercetin , antineoplastic activity , apoptosis , Article , cancer inhibition , cell aging , cell culture , cell growth , cell growth assay , cell proliferation , cell viability , COLO 320 cell line , COLO 741 cell line , colon adenocarcinoma cell line , colon cancer , controlled study , cytotoxicity , fetal bovine serum , human , human cell , immunocytochemistry , MTT assay , protein expression , TUNEL assay , apoptosis , cell aging , cell survival , chemical structure , chemistry , colon tumor , dose response , drug effect , drug screening , pathology , structure activity relation