Quercetin-mediated apoptosis and cellular senescence in human colon cancer

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dc.contributor.authorÖzsoy S.
dc.contributor.authorBecer E.
dc.contributor.authorKabadayı H.
dc.contributor.authorVatansever H.S.
dc.contributor.authorYücecan S.
dc.date.accessioned2024-07-22T08:07:59Z
dc.date.available2024-07-22T08:07:59Z
dc.date.issued2020
dc.description.abstractBackground: Quercetin is a flavonol from the flavonoid group of polyphenols, which positively affects human health due to its anti-cancer, anti-inflammatory, anti-microbial and cardioprotective effects. The effects of phenolic compounds, including quercetin, on programmed cell death and cellular senescence, have been the subject of research in recent years. Objective: In this study, we aimed to investigate the effects of quercetin on cell viability, apoptosis and cellular senescence in primary (Colo-320) and metastatic (Colo-741) colon adenocarcinoma cell lines. Methods: Cytotoxicity was analyzed via MTT assay in Colo-320 and Colo-741 cell lines. After quercetin treatment, cell ularsenescence and apoptosis were evaluated by TUNEL staining, X-Gal staining and indirect peroxidase tech-nique for immunocytochemical analysis of related proteins such as Bax, Bcl-2, caspase-3, Hsp27, Lamin B1, p16, cyclin B1. Results: The effective dose for inhibition of cell growth in both cell lines was determined to be 25µg/ml quercetin for 48 hours. Increased Baximmunoreactivityfollowingquercetin treatment was significant in both Colo-320 and Colo-741 cell lines, but decreased Bcl-2 immunoreactivitywas significant only in theColo-320 primary cell line. In addition, after quercetin administration, the number of TUNEL positive cells and, immunoreactivities for p16, Lamin B1 and cyclin B1 in both Colo-320 and Colo-741 cells increased. Conclusion: Our results suggest that quercetin may only induce apoptosis in primary colon cancer cells. Further-more, quercetin also triggered senescence in colon cancer cells, but some cells remained alive, suggesting that colon cancer cells might have escaped from senescence. © 2020 Bentham Science Publishers.
dc.identifier.DOI-ID10.2174/1871520620666200408082026
dc.identifier.issn18715206
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14194
dc.language.isoEnglish
dc.publisherBentham Science Publishers
dc.subjectAntineoplastic Agents
dc.subjectApoptosis
dc.subjectCell Proliferation
dc.subjectCell Survival
dc.subjectCells, Cultured
dc.subjectCellular Senescence
dc.subjectColonic Neoplasms
dc.subjectDose-Response Relationship, Drug
dc.subjectDrug Screening Assays, Antitumor
dc.subjectHumans
dc.subjectMolecular Structure
dc.subjectQuercetin
dc.subjectStructure-Activity Relationship
dc.subjectcaspase 3
dc.subjectcyclin B1
dc.subjectdiaminobenzidine
dc.subjectdistilled water
dc.subjectedetic acid
dc.subjectferric ferrocyanide
dc.subjectglutamine
dc.subjectheat shock protein 27
dc.subjecthematoxylin
dc.subjectlamin B
dc.subjectLamin B1
dc.subjectmagnesium chloride
dc.subjectparaformaldehyde
dc.subjectpenicillin derivative
dc.subjectperoxidase
dc.subjectphosphate buffered saline
dc.subjectprotein Bax
dc.subjectprotein bcl 2
dc.subjectprotein p16
dc.subjectquercetin
dc.subjectsodium chloride
dc.subjectstreptavidin
dc.subjectstreptomycin
dc.subjecttetrazolium
dc.subjecttriton x 100
dc.subjecttrypsin
dc.subjectunclassified drug
dc.subjectantineoplastic agent
dc.subjectquercetin
dc.subjectantineoplastic activity
dc.subjectapoptosis
dc.subjectArticle
dc.subjectcancer inhibition
dc.subjectcell aging
dc.subjectcell culture
dc.subjectcell growth
dc.subjectcell growth assay
dc.subjectcell proliferation
dc.subjectcell viability
dc.subjectCOLO 320 cell line
dc.subjectCOLO 741 cell line
dc.subjectcolon adenocarcinoma cell line
dc.subjectcolon cancer
dc.subjectcontrolled study
dc.subjectcytotoxicity
dc.subjectfetal bovine serum
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunocytochemistry
dc.subjectMTT assay
dc.subjectprotein expression
dc.subjectTUNEL assay
dc.subjectapoptosis
dc.subjectcell aging
dc.subjectcell survival
dc.subjectchemical structure
dc.subjectchemistry
dc.subjectcolon tumor
dc.subjectdose response
dc.subjectdrug effect
dc.subjectdrug screening
dc.subjectpathology
dc.subjectstructure activity relation
dc.titleQuercetin-mediated apoptosis and cellular senescence in human colon cancer
dc.typeArticle

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