Effectiveness of peptone-yeast extract (P-Y) medium in the cultivation and isolation of Entamoeba histolytica/Entamoeba dispar in Turkish patients
| dc.contributor.author | Dagci H. | |
| dc.contributor.author | Balcioglu I.C. | |
| dc.contributor.author | Ertabaklar H. | |
| dc.contributor.author | Kurt O. | |
| dc.contributor.author | Atambay M. | |
| dc.date.accessioned | 2025-04-10T11:17:53Z | |
| dc.date.available | 2025-04-10T11:17:53Z | |
| dc.date.issued | 2003 | |
| dc.description.abstract | Amebiasis is a common protozoan infection worldwide, causing serious health problems in both children and adults. Today, almost 10% of the world population is infected with Entamoeba histolytica/Entamoeba dispar. The aims of this study were both the comparison of the reproduction rates and densities of E. histolytica/E. dispar in Robinson, Dobell-Laidlaw and P-Y culture media and isolation of E. histolytica/E. dispar from stool samples in Peptone-Yeast (P-Y) medium. Trophozoites and cysts of E. histolytica/E. dispar, maintained in Robinson medium, and stool samples of patients with amebiasis were inoculated into P-Y, Robinson and Dobell-Laidlaw culture media. Reproduction rates reached their peak levels 48 h after the inoculation in all culture media. Reproduction rates in P-Y and Robinson media were found similar; however, they were higher than the reproduction rate in Dobell-Laidlaw medium (p < 0.01); there was no statistically significant difference between the reproduction rates of P-Y and Robinson media (p > 0.05). Twelve isolates from 12 patients were cultivated in P-Y medium and checked for reproduction everyday for 7 days. Twelve of the 12 (100%) isolates were cultivated in P-Y medium, indicating that the P-Y was an effective medium for the isolation of E. histolytica/E. dispar in stool samples. According to these results, P-Y medium could be preferred in immunologic, serologic and molecular studies and, thus the definitive diagnosis of amebiasis due to its low cost and simple formula. © 2003 Elsevier Science Inc. All rights reserved. | |
| dc.identifier.DOI-ID | 10.1016/S0732-8893(02)00508-4 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14701/52998 | |
| dc.title | Effectiveness of peptone-yeast extract (P-Y) medium in the cultivation and isolation of Entamoeba histolytica/Entamoeba dispar in Turkish patients | |
| dc.type | Article |