Comparative study on genotypic properties of Pseudomonas aeruginosa isolated from subclinical mastitis in T?rkiye
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The results of antibacterial treatment in mastitis may be related not only to the antibiotic sensitivity of the etiological factors but also to their abilities to carry integron and virulence genes. Integrons are mobile DNA elements that can capture and carry genes, particularly those responsible for antibiotic resistance. Pseudomonas aeru-ginosa has numberless virulence factors which are contributed to bacterial invasion and toxicity. This study aims to investigate antibiotic resistance, integron and virulence gene profiles of P. aeruginosa isolates obtained from cow milk with subclinical mastitis; further, was to evaluate the relationship between the antimicrobial resistance of bacteria with the integron and virulence gene carrying. The material of the study consists of 32 (9.8%) P. aeruginosa isolates ob-tained from 326 subclinical mastitis milk samples. After the bacterial identification by classical conventional methods, polymerase chain reaction (PCR) was carried out to confirm the genus and species of the isolates and to determine the integron and virulence gene profiles. Antimicrobial resistance was determined by the disk diffusion method using fifteen antibiotics belonging to eleven antimicrobial families. The relationship between the presence of integron and virulence genes associated with antibiotic resistance, further the relationship between the presence of virulence genes and antimicrobial resistance was calculated with the Chi-Square (chi 2) test. Ten virulence genes (lasl, lasR, lasB, rhll, rhlR, rhlAB, plcH, plcN, ppyR, exoT) were found in all isolates whilst another virulence gene (aprA) was not present in any isolate. It was found that 34.4% of the isolates carried any integron gene. The results showed that the relation-ship is important between the presence of int genes and gentamicin, amikacin, tetracycline, cefoperazone, imipenem, aztreonam, ciprofloxacin, enrofloxacin resistance and exoU virulence gene presence. Also; there were also important associations between resistance to certain antibiotics and the presence of P. aeruginosa virulence genes. All isolates obtained in this study showed multiple antibiotic resistance (MDR). In these cases, showing the presence of integron and some virulence genes could play a prominent role in the resistance of P. aeruginosa isolates to antimicrobial drugs. This study may be important as it is the first study to show the presence of antibiotic resistance, integron and virulence genes together in subclinical mastitis cow's milk isolates of P. aeruginosa in Turkiye. The presence of antibiotic -re-sistant P. aeruginosa strains in cattle farms may also pose a public health risk, as these bacteria can transmit their resistance genes to humans through food consumption.