Expression profiling of stem cell signaling alters with spheroid formation in CD133high/CD44high prostate cancer stem cells

Simple item page
dc.contributor.authorOktem, G
dc.contributor.authorBilir, A
dc.contributor.authorUslu, R
dc.contributor.authorInan, SV
dc.contributor.authorDemiray, SB
dc.contributor.authorAtmaca, H
dc.contributor.authorAyla, S
dc.contributor.authorSercan, O
dc.contributor.authorUysal, A
dc.date.accessioned2024-07-18T12:06:28Z
dc.date.available2024-07-18T12:06:28Z
dc.description.abstractCancer stem cells (CSC) isolated from multiple tumor types differentiate in vivo and in vitro when cultured in serum; however, the factors responsible for their differentiation have not yet been identified. The first aim of the present study was to identify CD133(high)/CD44(high) DU145 prostate CSCs and compare their profiles with non-CSCs as bulk counterparts of the population. Subsequently, the two populations continued to be three-dimensional multicellular spheroids. Differentiation was then investigated with stem cell-related genomic characteristics. Polymerase chain reaction array analyses of cell cycle regulation, embryonic and mesenchymal cell lineage-related markers, and telomerase reverse transcriptase (TERT) and Notch signaling were performed. Immunohistochemistry of CD117, Notch1, Jagged1, Delta1, Sox2, c-Myc, Oct4, KLF4, CD90 and SSEA1 were determined in CSC and non-CSC monolayer and spheroid subcultures. Significant gene alterations were observed in the CD133(high)/CD44(high) population when cultured as a monolayer and continued as spheroid. In this group, marked gene upregulation was determined in collagen type 9 a1, Islet1 and cyclin D2. Jagged1, Delta-like 3 and Notch1 were respectively upregulated genes in the Notch signaling pathway. According to immunoreactivity, the staining density of Jagged1, Sox2, Oct4 and Klf-4 increased significantly in CSC spheroids. Isolated CSCs alter their cellular characterization over the course of time and exhibit a differentiation profile while maintaining their former surface antigens at a level of transcription or translation. The current study suggested that this differentiation process may be a mechanism responsible for the malignant process and tumor growth.
dc.identifier.issn1792-1074
dc.identifier.other1792-1082
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/10413
dc.language.isoEnglish
dc.publisherSPANDIDOS PUBL LTD
dc.subjectHEPATOCELLULAR-CARCINOMA CELLS
dc.subjectJAGGED1 EXPRESSION
dc.subjectGENE-EXPRESSION
dc.subjectBREAST-CANCER
dc.subjectHYPERMETHYLATION
dc.subjectCD133(+)
dc.subjectIDENTIFICATION
dc.subjectHETEROGENEITY
dc.subjectANGIOGENESIS
dc.subjectMOLECULES
dc.titleExpression profiling of stem cell signaling alters with spheroid formation in CD133high/CD44high prostate cancer stem cells
dc.typeArticle

Files

Collections

Web of Science Koleksiyonu