Low Mononuclear Cell IL-18 and IL-27 Response in Children: Susceptibility to Tuberculosis Infection after Contact

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dc.contributor.authorKarabacak, HA
dc.contributor.authorYilmaz, O
dc.contributor.authorTuglu, I
dc.contributor.authorTaneli, F
dc.contributor.authorSurucuoglu, S
dc.contributor.authorKanik, ET
dc.contributor.authorOzkutuk, N
dc.contributor.authorGozukara, C
dc.contributor.authorOzkut, MM
dc.contributor.authorTurkeli, A
dc.contributor.authorYuksel, H
dc.date.accessioned2024-07-18T11:49:12Z
dc.date.available2024-07-18T11:49:12Z
dc.description.abstractBackground Identification of the immune response against tuberculosis is vital to develop new diagnostic and therapeutic modalities. The objective of this study was to determine IL (interleukin)-18 and IL-27 responses of peripheral blood mononuclear cells to early secreted antigen (ESAT-6) and culture filtrate protein-10 (CFP-10) stimulation in children with a (+) or (-) tuberculin skin test (TST) with in-house tuberculosis contact. Methods We enrolled 40 children aged 1 to 5 years who had an in-house contact with a tuberculous adult. Blood samples were obtained from all children for QuantiFERON tuberculosis (TB) gold in tube (QFT-GIT), and peripheral blood mononuclear blood cell culture tests. The subjects were grouped as TST (-) QFT-GIT (-), TST (+) QFT-GIT (-), and TST (+) QFT-GIT (+). Supernatant of peripheral blood mononuclear cell culture was separated with and without stimulation of ESAT-6 and CFP-10, and IL-18 and IL-27 levels were measured with enzyme linked immunoassay (ELISA) test. Results The study group included 22 boys and 18 girls with mean age 4.25 +/- 0.9 years. IL-18 and IL-27 levels were statistically significant in ESAT-6/CFP-10-stimulated supernatants of peripheral blood mononuclear cell (PBMC) samples among the three groups (p = 0.000,p = 0.007, respectively). IL-18 levels between the TST (-) QFT-GIT (-) and TST (+) QFT-GIT (+) groups were significantly different (p = 0.026). Both IL-18 and IL-27 levels were significantly different between ESAT-6/CFP-10 stimulated PBMC supernatants of TST (-) QFT-GIT (-) and TST (+) QFT-GIT (-) groups (p = 0.000,p = 0.003, respectively). Conclusion Low IL-18 and IL-27 responses of peripheral blood mononuclear cells in children with Bacillus Calmette-Guerin (BCG) vaccine may play a role inMycobacterium tuberculosisinfection after in-house contact.
dc.identifier.issn1305-7707
dc.identifier.other1305-7693
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/3850
dc.language.isoEnglish
dc.publisherGEORG THIEME VERLAG KG
dc.subjectINTERFERON-GAMMA
dc.subjectINTERLEUKIN-18
dc.subjectIMMUNITY
dc.subjectGROWTH
dc.titleLow Mononuclear Cell IL-18 and IL-27 Response in Children: Susceptibility to Tuberculosis Infection after Contact
dc.typeArticle

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