Effects of adipose and bone marrow-derived mesenchymal stem cells on vaginal atrophy in a rat menopause model

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dc.contributor.authorKasap B.
dc.contributor.authorKasap
dc.contributor.authorVatansever S.
dc.contributor.authorKendirci R.
dc.contributor.authorYılmaz O.
dc.contributor.authorÇalışır M.
dc.contributor.authorEdgünlü T.
dc.contributor.authorAkın M.N.
dc.date.accessioned2024-07-22T08:08:28Z
dc.date.available2024-07-22T08:08:28Z
dc.date.issued2019
dc.description.abstractBackground & objectives: Vaginal atrophy is characterized by thinning of vaginal epithelial layers and decreased local blood flow. We aimed to evaluate the regenerative effects of Adipose derived mesenchymal stem cells (ADMSC) and Bone marrow derived mesenchymal stem cells (BMDSC) on vaginal atrophy in rat menopause model. Materials and methods: Rats were randomly divided into 4 (four) groups: sham, control, ADMSC, BMDSC. Vaginal epithelial thickness, structure of the lamina propria, blood vessels in the lamina propria, collagen deposition, and muscle structure were evaluated. Anti ER α, VEGF, VEGFR 1, Bax and bcl-2 antibodies were analyzed. Beta actin gene was used as endogenous control. Genetical differences among the groups were compared by using Kruskal Wallis and Mann Whitney U test. p < 0.05 was regarded as statistically significant. Results: Epithelial thickness of ADMSC group was higher than control group, but less than sham group Epithelial thickness of BMDSC group was less than sham group. Lamina propria and muscle tissue of ADMSC and BMDSC groups were found to be similar to sham group. VEGFR-1, VEGF, Bax and ER-α staining levels were higher in ADMSC and BMDSC groups than control group. ADMSC group stained stronger with VEGFR-1 and VEGF than BMDSC group. Bcl-2 staining level was increased in ADMSC applied group. No statistically significant difference was detected in Bax and Bcl-2 genes and Bax-/Bcl-2 ratio. Conclusions: Although genetic expression might have ended and could not be significantly demonstrated, histological and immunohistochemical results favor ADMSC application in vaginal atrophy rather than BMDSC. © 2019 Elsevier B.V.
dc.identifier.DOI-ID10.1016/j.gene.2019.06.027
dc.identifier.issn03781119
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14399
dc.language.isoEnglish
dc.publisherElsevier B.V.
dc.subjectAdipose Tissue
dc.subjectAnimals
dc.subjectAtrophy
dc.subjectbcl-2-Associated X Protein
dc.subjectBiomarkers
dc.subjectBone Marrow Cells
dc.subjectCells, Cultured
dc.subjectDisease Models, Animal
dc.subjectEstrogen Receptor alpha
dc.subjectFemale
dc.subjectHumans
dc.subjectMenopause
dc.subjectMesenchymal Stem Cell Transplantation
dc.subjectMesenchymal Stem Cells
dc.subjectRats
dc.subjectVagina
dc.subjectVascular Endothelial Growth Factor A
dc.subjectVascular Endothelial Growth Factor Receptor-1
dc.subjectbeta actin
dc.subjectestrogen receptor alpha
dc.subjectprotein Bax
dc.subjectprotein bcl 2
dc.subjectvasculotropin
dc.subjectvasculotropin receptor 1
dc.subjectBax protein, mouse
dc.subjectbiological marker
dc.subjectestrogen receptor alpha
dc.subjectprotein Bax
dc.subjectvascular endothelial growth factor A, mouse
dc.subjectvasculotropin A
dc.subjectvasculotropin receptor 1
dc.subjectadipose derived mesenchymal stem cell
dc.subjectadipose tissue cell
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectBax gene
dc.subjectBcl 2 gene
dc.subjectbeta actin gene
dc.subjectblood vessel
dc.subjectbone marrow derived mesenchymal stem cell
dc.subjectcollagen metabolism
dc.subjectcontrolled study
dc.subjectfemale
dc.subjectgene expression
dc.subjecthistopathology
dc.subjectimmunohistochemistry
dc.subjectlamina propria
dc.subjectmenopause
dc.subjectmesenchymal stem cell
dc.subjectmuscle tissue
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectrat
dc.subjectrat model
dc.subjectvagina atrophy
dc.subjectvagina epithelium
dc.subjectWistar rat
dc.subjectadipose tissue
dc.subjectanimal
dc.subjectatrophy
dc.subjectbone marrow cell
dc.subjectcell culture
dc.subjectcytology
dc.subjectdisease model
dc.subjecthuman
dc.subjectmenopause
dc.subjectmesenchymal stem cell
dc.subjectmesenchymal stem cell transplantation
dc.subjectmetabolism
dc.subjectpathology
dc.subjectphysiology
dc.subjectprocedures
dc.subjectvagina
dc.titleEffects of adipose and bone marrow-derived mesenchymal stem cells on vaginal atrophy in a rat menopause model
dc.typeArticle

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