In-vitro Evaluation of Effects of Mesenchymal Stem Cells on TLR3, TLR7/8 and TLR9-activated Natural Killer Cells

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dc.contributor.authorOzdemir, AT
dc.contributor.authorKirmaz, C
dc.contributor.authorOzdemir, RBO
dc.contributor.authorDegirmenci, P
dc.contributor.authorOztatlici, M
dc.contributor.authorDegirmenci, M
dc.date.accessioned2024-07-18T12:00:54Z
dc.date.available2024-07-18T12:00:54Z
dc.description.abstractObjectives: In this study, it was aimed to investigate the immunomodulatory effects of Mesenchymal stem cells (MSCs) on Natural Killer (NK) cells activated by Toll-like receptor (TLR) agonists. Methods: MDA-MB-231, MCF-7 and NK-92 cells were induced with TLR3, TLR7/8 and TLR9 agonists and co-cultured with MSCs. Alterations in IFN-gamma, TNF-alpha, Granzyme-b and Perforin expressions were determined by qPCR method, CD69 and CD107a expressions were determined by flow cytometry, and cytotoxicity was determined by MTT-assay. Results: All TLR agonists significantly increased the expressions of the IFN-gamma, TNF-alpha, Granzyme-b, Perforin, CD69 and CD107a in-vitro. We determined that the cytokine, cytotoxic molecules, and activation markers of NK-92 cells interacting with breast tumor cells significantly increased by TLR3 and TLR9 agonists. However, suppression rather than activation occurred on the NK-92 cells due to the simultaneous induction of the immunosuppressive effects of MSCs by these agonists. On the other hand, the TLR7/8 agonists provided a low NK-92 induction, however, the inhibitory effects of MSCs were not triggered. Therefore, it provided a more significant activation than TLR3 and TLR9 agonists. Conclusion: Our findings suggested that TLR7/8 agonists may be a better choice to induce antitumor effects of NK cells in a tumor tissue rich in MSCs.
dc.identifier.other2587-196X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/8058
dc.language.isoEnglish
dc.publisherKARE PUBL
dc.subjectTOLL-LIKE RECEPTORS
dc.subjectLIGANDS
dc.titleIn-vitro Evaluation of Effects of Mesenchymal Stem Cells on TLR3, TLR7/8 and TLR9-activated Natural Killer Cells
dc.typeArticle

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