The effect of radiolabeled antibiotics on biofilm and microorganism within biofilm
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Date
2018
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Abstract
The aim of this study was to investigate the 131I and 127I labeled linezolid and moxifloxacin effects of minimum inhibitory concentration, and minimum bactericidal concentration on mature biofilm and microorganism within the biofilm. Linezolid and moxifloxacin were labeled with 131I and 127I and chromatography studies were carried out with thin layer radiochromatograpy and high-pressure liquid radiochromatography techniques. Specific activities of radiolabeled LZD and MXF was found to be 53.3 ± 3.1 and 127.3 ± 1.1 MBq/µmol for [131I]LZD and 7.6 ± 0.02 and 55.6 ± 0.8 MBq/µmol for [131I]MXF, respectively. The minimum inhibitory concentration and Time-Kill of Linezolid and moxifloxacin alone and their 131I and 127I labeled forms were tested with a standard strain of meticillin-susceptible Staphylocıccus aureus. MIC values of LNZ and MXF were 2.96 nmol/mL (1 µg/ml) and 0.141 nmol/mL (0.062 µg/ml). Time Kills of MXF and LZD were found to be 0.06 and 1 μg, respectively. Antibiotics labeled with beta-emitting radioactive molecule may be a new theranostics strategy for biofilm infections. © 2018, Akadémiai Kiadó, Budapest, Hungary.
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Keywords
iodine , iodine 131 , linezolid , moxifloxacin , Article , bacterial strain , biofilm , controlled study , drug determination , drug structure , high performance liquid chromatography , isotope labeling , methicillin susceptible Staphylococcus aureus , minimum bactericidal concentration , minimum inhibitory concentration , nonhuman , Staphylococcus aureus , theranostic nanomedicine , thin layer chromatography