The effect of radiolabeled antibiotics on biofilm and microorganism within biofilm

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dc.contributor.authorAvcıbaşı U.
dc.contributor.authorDemiroğlu H.
dc.contributor.authorSakarya S.
dc.contributor.authorÜnak P.
dc.contributor.authorTekin V.
dc.contributor.authorAteş B.
dc.date.accessioned2024-07-22T08:09:56Z
dc.date.available2024-07-22T08:09:56Z
dc.date.issued2018
dc.description.abstractThe aim of this study was to investigate the 131I and 127I labeled linezolid and moxifloxacin effects of minimum inhibitory concentration, and minimum bactericidal concentration on mature biofilm and microorganism within the biofilm. Linezolid and moxifloxacin were labeled with 131I and 127I and chromatography studies were carried out with thin layer radiochromatograpy and high-pressure liquid radiochromatography techniques. Specific activities of radiolabeled LZD and MXF was found to be 53.3 ± 3.1 and 127.3 ± 1.1 MBq/µmol for [131I]LZD and 7.6 ± 0.02 and 55.6 ± 0.8 MBq/µmol for [131I]MXF, respectively. The minimum inhibitory concentration and Time-Kill of Linezolid and moxifloxacin alone and their 131I and 127I labeled forms were tested with a standard strain of meticillin-susceptible Staphylocıccus aureus. MIC values of LNZ and MXF were 2.96 nmol/mL (1 µg/ml) and 0.141 nmol/mL (0.062 µg/ml). Time Kills of MXF and LZD were found to be 0.06 and 1 μg, respectively. Antibiotics labeled with beta-emitting radioactive molecule may be a new theranostics strategy for biofilm infections. © 2018, Akadémiai Kiadó, Budapest, Hungary.
dc.identifier.DOI-ID10.1007/s10967-018-5750-3
dc.identifier.issn02365731
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15015
dc.language.isoEnglish
dc.publisherSpringer Netherlands
dc.subjectiodine
dc.subjectiodine 131
dc.subjectlinezolid
dc.subjectmoxifloxacin
dc.subjectArticle
dc.subjectbacterial strain
dc.subjectbiofilm
dc.subjectcontrolled study
dc.subjectdrug determination
dc.subjectdrug structure
dc.subjecthigh performance liquid chromatography
dc.subjectisotope labeling
dc.subjectmethicillin susceptible Staphylococcus aureus
dc.subjectminimum bactericidal concentration
dc.subjectminimum inhibitory concentration
dc.subjectnonhuman
dc.subjectStaphylococcus aureus
dc.subjecttheranostic nanomedicine
dc.subjectthin layer chromatography
dc.titleThe effect of radiolabeled antibiotics on biofilm and microorganism within biofilm
dc.typeArticle

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