The protective effect of granulocyte colony-stimulating factor on endometrium and ovary in a rat model of diabetes mellitus
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2014
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Abstract
Aims: To evaluate the effect of granulocyte colony-stimulating factor (G-CSF) on the endometrium and ovaries in an experimental diabetes mellitus (DM) rat model. Methods: A total of 18 female Sprague-Dawley albino mature rats (8 weeks, 200-220 g) were used in this study. Diabetes was induced by intraperitoneal (i.p.) injection of streptozocin randomly in 12 rats. No drug was administered to the remainder of the rats (control group, group 1, n = 6). The other 12 rats were randomly divided into 2 groups; 1 ml/kg i.p. saline was given as vehicle to group 2 (diabetic nontreated control group, n = 6) and 100 μg/kg/day of i.p. G-CSF was given to group 3 (G-CSF-treated group, n = 6) for 4 weeks. After 4 weeks, blood samples were collected and hysterectomy with bilateral oophorectomy was performed for histopathological examination. Results: The mean endometrial gland degeneration and stromal fibrosis scores were significantly higher in group 2 compared with groups 1 and 3. Ovarian follicle degeneration, stromal degeneration and stromal fibrosis scores were significantly higher in group 2 compared with groups 1 and 3. Plasma TGF-β and malondialdehyde levels were significantly lower in groups 1 and 3 compared with group 2. Antimüllerian hormone levels were significantly lower in group 2 compared with groups 1 and 3. Conclusion: Glucose toxicity occurred severely in the ovaries and endometrium of the DM rats. After G-CSF treatment, ovarian and endometrial injury and fibrosis scores decreased significantly. The effects of G-CSF in rat models give hope to improved treatment of human DM complications such as premature ovarian failure and endometrial dysfunction. © 2014 S. Karger AG, Basel.
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Animals , Diabetes Mellitus, Experimental , Endometrium , Female , Granulocyte Colony-Stimulating Factor , Malondialdehyde , Ovarian Diseases , Ovary , Primary Ovarian Insufficiency , Rats , Rats, Sprague-Dawley , Transforming Growth Factor beta , Uterine Diseases , glucose , granulocyte colony stimulating factor , malonaldehyde , Muellerian inhibiting factor , recombinant granulocyte colony stimulating factor , streptozocin , thiobarbituric acid reactive substance , transforming growth factor beta , trichloroacetic acid , filgrastim , sodium chloride , granulocyte colony stimulating factor , malonaldehyde , transforming growth factor beta , animal experiment , animal model , animal tissue , article , controlled study , degeneration , drug effect , endometrial disease , endometrial gland degeneration , endometrial stromal fibrosis , female , fibrosis , glucose blood level , glucotoxicity , histopathology , hysterectomy , injury severity , lipid peroxidation , nonhuman , ovarian follicle degeneration , ovarian stromal fibrosis , ovariectomy , ovary disease , ovary follicle , oxidative stress , priority journal , protein blood level , rat , streptozotocin-induced diabetes mellitus , stromal degeneration , Article , blood sampling , endometrium , experimental diabetes mellitus , ovary , tissue degeneration , animal , blood , complication , endometrium , experimental diabetes mellitus , Ovarian Diseases , ovary , pathology , Primary Ovarian Insufficiency , Sprague Dawley rat , Uterine Diseases