The protective effect of granulocyte colony-stimulating factor on endometrium and ovary in a rat model of diabetes mellitus

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dc.contributor.authorPala H.G.
dc.contributor.authorPala E.E.
dc.contributor.authorArtunc Ulkumen B.
dc.contributor.authorAktug H.
dc.contributor.authorYavasoglu A.
dc.contributor.authorKorkmaz H.A.
dc.contributor.authorErbas O.
dc.date.accessioned2024-07-22T08:16:51Z
dc.date.available2024-07-22T08:16:51Z
dc.date.issued2014
dc.description.abstractAims: To evaluate the effect of granulocyte colony-stimulating factor (G-CSF) on the endometrium and ovaries in an experimental diabetes mellitus (DM) rat model. Methods: A total of 18 female Sprague-Dawley albino mature rats (8 weeks, 200-220 g) were used in this study. Diabetes was induced by intraperitoneal (i.p.) injection of streptozocin randomly in 12 rats. No drug was administered to the remainder of the rats (control group, group 1, n = 6). The other 12 rats were randomly divided into 2 groups; 1 ml/kg i.p. saline was given as vehicle to group 2 (diabetic nontreated control group, n = 6) and 100 μg/kg/day of i.p. G-CSF was given to group 3 (G-CSF-treated group, n = 6) for 4 weeks. After 4 weeks, blood samples were collected and hysterectomy with bilateral oophorectomy was performed for histopathological examination. Results: The mean endometrial gland degeneration and stromal fibrosis scores were significantly higher in group 2 compared with groups 1 and 3. Ovarian follicle degeneration, stromal degeneration and stromal fibrosis scores were significantly higher in group 2 compared with groups 1 and 3. Plasma TGF-β and malondialdehyde levels were significantly lower in groups 1 and 3 compared with group 2. Antimüllerian hormone levels were significantly lower in group 2 compared with groups 1 and 3. Conclusion: Glucose toxicity occurred severely in the ovaries and endometrium of the DM rats. After G-CSF treatment, ovarian and endometrial injury and fibrosis scores decreased significantly. The effects of G-CSF in rat models give hope to improved treatment of human DM complications such as premature ovarian failure and endometrial dysfunction. © 2014 S. Karger AG, Basel.
dc.identifier.DOI-ID10.1159/000363239
dc.identifier.issn03787346
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/16903
dc.language.isoEnglish
dc.publisherS. Karger AG
dc.subjectAnimals
dc.subjectDiabetes Mellitus, Experimental
dc.subjectEndometrium
dc.subjectFemale
dc.subjectGranulocyte Colony-Stimulating Factor
dc.subjectMalondialdehyde
dc.subjectOvarian Diseases
dc.subjectOvary
dc.subjectPrimary Ovarian Insufficiency
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectTransforming Growth Factor beta
dc.subjectUterine Diseases
dc.subjectglucose
dc.subjectgranulocyte colony stimulating factor
dc.subjectmalonaldehyde
dc.subjectMuellerian inhibiting factor
dc.subjectrecombinant granulocyte colony stimulating factor
dc.subjectstreptozocin
dc.subjectthiobarbituric acid reactive substance
dc.subjecttransforming growth factor beta
dc.subjecttrichloroacetic acid
dc.subjectfilgrastim
dc.subjectsodium chloride
dc.subjectgranulocyte colony stimulating factor
dc.subjectmalonaldehyde
dc.subjecttransforming growth factor beta
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectdegeneration
dc.subjectdrug effect
dc.subjectendometrial disease
dc.subjectendometrial gland degeneration
dc.subjectendometrial stromal fibrosis
dc.subjectfemale
dc.subjectfibrosis
dc.subjectglucose blood level
dc.subjectglucotoxicity
dc.subjecthistopathology
dc.subjecthysterectomy
dc.subjectinjury severity
dc.subjectlipid peroxidation
dc.subjectnonhuman
dc.subjectovarian follicle degeneration
dc.subjectovarian stromal fibrosis
dc.subjectovariectomy
dc.subjectovary disease
dc.subjectovary follicle
dc.subjectoxidative stress
dc.subjectpriority journal
dc.subjectprotein blood level
dc.subjectrat
dc.subjectstreptozotocin-induced diabetes mellitus
dc.subjectstromal degeneration
dc.subjectArticle
dc.subjectblood sampling
dc.subjectendometrium
dc.subjectexperimental diabetes mellitus
dc.subjectovary
dc.subjecttissue degeneration
dc.subjectanimal
dc.subjectblood
dc.subjectcomplication
dc.subjectendometrium
dc.subjectexperimental diabetes mellitus
dc.subjectOvarian Diseases
dc.subjectovary
dc.subjectpathology
dc.subjectPrimary Ovarian Insufficiency
dc.subjectSprague Dawley rat
dc.subjectUterine Diseases
dc.titleThe protective effect of granulocyte colony-stimulating factor on endometrium and ovary in a rat model of diabetes mellitus
dc.typeArticle

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