Metal-chelated cryogels for amyloglucosidase adsorption: application for continuous starch hydrolysis

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dc.contributor.authorEvli, S
dc.contributor.authorOrhan, H
dc.contributor.authorAktas, PS
dc.contributor.authorUygun, M
dc.contributor.authorUygun, DA
dc.date.accessioned2024-07-18T11:53:37Z
dc.date.available2024-07-18T11:53:37Z
dc.description.abstractIn the present work, a new metal-chelating platform was designed by using IDA as a chelating agent and Cu(II) as an affinity component for amyloglucosidase adsorption. Poly(AAm-GMA) cryogels were used as structural elements, while GMA monomer served reactive epoxy groups for IDA immobilization. Synthesized cryogels were characterized by FTIR, SEM and EDX studies. Pore diameter of the whole polymeric structure was 3-10 Effects of medium pH, temperature, ionic strength along with amyloglucosidase concentration were also investigated for more effective amyloglucosidase adsorption and maximum adsorbed amount of amyloglucosidase was cryogel by the optimum conditions. Reusability profile of the poly(AAm-GMA)-IDA-cryogels was also studied and it was found that the synthesized cryogels could be used repeatedly for many times without any significant decrease on their adsorption capacity. Also continuous hydrolysis of starch by using immobilized form of amyloglucosidase in a column system was studied.
dc.identifier.issn0250-4707
dc.identifier.other0973-7669
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/5746
dc.language.isoEnglish
dc.publisherINDIAN ACAD SCIENCES
dc.subjectREVERSIBLE IMMOBILIZATION
dc.subjectAFFINITY-CHROMATOGRAPHY
dc.subjectMONOLITHIC CRYOGELS
dc.subjectFERMENTATION BROTH
dc.subjectGLUCOAMYLASE
dc.subjectPURIFICATION
dc.subjectPROTEINS
dc.subjectCAPTURE
dc.subjectENZYME
dc.titleMetal-chelated cryogels for amyloglucosidase adsorption: application for continuous starch hydrolysis
dc.typeArticle

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