The effect of dietary and topical celecoxib on 4-nitroquinoline-1-oxide- induced lingual epithelium alternations in rat
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Date
2009
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Abstract
Objective: To compare the preventive effect of dietary and topical Celecoxib (CCB), a potent inhibitor of COX-2 on 4-NQO-induced tongue SCC in rat. Methods: Fifty male Sprague Dawley adult 3- 3.5 months old rats were used as animal model in this study. The tongue SCC was induced by a daily administration of 30 ppm 4-NQO, in drinking water, for 8 months. The rats in case groups received dietary or topical CCB. Tongue Specimens were prepared for histopathological and immunohistochemical (Ki-67) staining and or TUNEL assay were examined. Values are expressed as mean ± SEM and analyzed with Npar Kruscal Wallis and one-way ANOVA tests. p < 0.05 was considered significant. Results: The incidence of tongue precancer lesions, judged by morphological and morhometrical criteria and apoptosis/proliferation ratio, was significantly (p<0.01) reduced by CCB. The effect of topical CCB use, at high doses, was comparable to the effect of dietary CCB. Conclusion: Both topical and dietary CCB have inhibitory effect on 4-NQO induced SCC on tongue. The effect of CCB is probably mediated by suppression of cell proliferation and induction of apoptosis.
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Keywords
4-Nitroquinoline-1-oxide , Administration, Topical , Analysis of Variance , Animals , Cyclooxygenase Inhibitors , Diet , Disease Models, Animal , In Situ Nick-End Labeling , Male , Precancerous Conditions , Pyrazoles , Rats , Rats, Sprague-Dawley , Sulfonamides , Tongue , Tongue Neoplasms , 4 nitroquinoline 1 oxide , celecoxib , drinking water , aged , animal experiment , animal model , animal tissue , apoptosis , article , cancer incidence , cell proliferation , controlled study , drug effect , fluid intake , food intake , histopathology , immunohistochemistry , male , nick end labeling , nonhuman , rat , scanning electron microscopy , tongue carcinoma