Multicenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis

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dc.contributor.authorCoban, AY
dc.contributor.authorAkbal, AU
dc.contributor.authorBicmen, C
dc.contributor.authorAlbay, A
dc.contributor.authorSig, AK
dc.contributor.authorUzun, M
dc.contributor.authorSelale, DS
dc.contributor.authorOzkutuk, N
dc.contributor.authorSurucuoglu, S
dc.contributor.authorAlbayrak, N
dc.contributor.authorUcarman, N
dc.contributor.authorOzkutuk, A
dc.contributor.authorEsen, N
dc.contributor.authorCeyhan, I
dc.contributor.authorOzyurt, M
dc.contributor.authorBektore, B
dc.contributor.authorAslan, G
dc.contributor.authorDelialioglu, N
dc.contributor.authorAlp, A
dc.date.accessioned2024-07-18T12:07:45Z
dc.date.available2024-07-18T12:07:45Z
dc.description.abstractThe aim of this multicenter study was to evaluate the performance of the crystal violet decolorization assay (CVDA) for detection of multidrug resistant tuberculosis (MDR-TB). This study was performed in 11 centers in two phases. A total of 156 isolates were tested for INH and RIF resistance. In the phase I, 106 clinical isolates were tested in the Center 1-7. In the phase 2, 156 clinical isolates were tested in the center 1-6, center 8-11. Eighty six of 156 tested isolates were the same in phase I. Agreements were 96.2-96.8% for INH and 98.1-98.7% for RIF in the phase I-II, respectively. Mean time to obtain the results in the phase I was 14.3 +/- 5.4 days. In the phase II, mean time to obtain the results was 11.6 +/- 3.5 days. Test results were obtained within 14days for 62.3% (66/106) of isolates in the phase I and 81.4% (127/156) of isolates in the phase II. In conclusion, CVDA is rapid, reliable, inexpensive, and easy to perform for rapid detection of MDR-TB isolates. In addition, it could be adapted for drug susceptibility testing with all drugs both in developed and developing countries.
dc.identifier.issn2045-2322
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/10620
dc.language.isoEnglish
dc.publisherNATURE PORTFOLIO
dc.subjectNITRATE REDUCTASE ASSAY
dc.subjectRESAZURIN MICROTITER ASSAY
dc.subjectMALACHITE GREEN
dc.subjectSTREPTOMYCIN RESISTANCE
dc.subjectMULTIDRUG-RESISTANT
dc.subjectSUSCEPTIBILITY
dc.subjectETHAMBUTOL
dc.subjectPLATE
dc.titleMulticenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis
dc.typeArticle

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