Effect of portal vein occlusion on the pancreas: An experimental model

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dc.contributor.authorAydede H.
dc.contributor.authorErhan Y.
dc.contributor.authorIkgül O.
dc.contributor.authorCilaker S.
dc.contributor.authorSakarya A.
dc.contributor.authorVatansever S.
dc.date.accessioned2024-07-22T08:23:25Z
dc.date.available2024-07-22T08:23:25Z
dc.date.issued2006
dc.description.abstractBackground: The effects of portal vein occlusion on the pancreas are not clearly understood. Therefore, we studied histomorphological changes induced in the rat pancreas by various periods of portal vein occlusion. Materials and methods: Sixty female Wistar albino rats were randomly allocated into four groups of 15 each. In Group I (control), rats underwent sham laparotomy to expose the portal vein proximal to its bifurcation. In Groups II-IV, rats underwent laparotomy followed by portal vein occlusion by clamping for 15, 30, and 60 minutes respectively. The pancreas was removed immediately after sham laparotomy in Group I and immediately after clamp release in Groups II-IV. Pancreatic tissue specimens were subjected to histochemical analysis for cell typing and diagnosis, immunohistochemical analysis for identification of the inflammatory markers tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), endothelial nitric oxide synthase (eNOS), and inducible NOS (iNOS), and TUNEL analysis was carried out for identification of apoptotic cells. Results: Histochemistry revealed signs of inflammation in pancreatic tissue from rats subjected to portal vein occlusion. Immunohistochemistry revealed that the expression of proinflammatory cytokines TNF-α and IL-1β and the oxidative damage indicator iNOS in rat pancreatic tissue increased progressively with the duration of portal vein occlusion. TUNEL assay revealed no signs of apoptosis in any of the groups. Conclusion: We conclude that portal vein occlusion triggers an inflammatory response in the pancreas that worsens the longer the occlusion lasts. © 2006 by the Société Internationale de Chirurgie.
dc.identifier.DOI-ID10.1007/s00268-005-7883-0
dc.identifier.issn14322323
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19527
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectConstriction, Pathologic
dc.subjectFemale
dc.subjectImmunohistochemistry
dc.subjectIn Situ Nick-End Labeling
dc.subjectInterleukin-1beta
dc.subjectNitric Oxide Synthase Type II
dc.subjectNitric Oxide Synthase Type III
dc.subjectPancreas
dc.subjectPancreatitis
dc.subjectPortal Vein
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectTumor Necrosis Factor-alpha
dc.subjectcytokine
dc.subjectendothelial nitric oxide synthase
dc.subjectinducible nitric oxide synthase
dc.subjectinterleukin 1beta
dc.subjecttumor necrosis factor alpha
dc.subjectacute pancreatitis
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectapoptosis
dc.subjectarticle
dc.subjectcell type
dc.subjectcontrolled study
dc.subjectcytokine production
dc.subjectfemale
dc.subjecthistochemistry
dc.subjecthistopathology
dc.subjectimmunohistochemistry
dc.subjectinflammation
dc.subjectlaparotomy
dc.subjectnick end labeling
dc.subjectnonhuman
dc.subjectoxidative stress
dc.subjectpancreas
dc.subjectportal vein obstruction
dc.subjectrandomization
dc.subjectrat
dc.subjectsham feeding
dc.subjecttissue section
dc.titleEffect of portal vein occlusion on the pancreas: An experimental model
dc.typeArticle

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