Investigation of the effects of rapamycin on the mTOR pathway and apoptosis in metastatic and non-metastatic human breast cancer cell lines

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dc.contributor.authorEkizceli, G
dc.contributor.authorUluer, ET
dc.contributor.authorInan, S
dc.date.accessioned2024-07-18T12:01:10Z
dc.date.available2024-07-18T12:01:10Z
dc.description.abstractAIM: The aim of this study was to analyze the effects of rapamycin treatment on apoptosis via mTOR pathway in metastatic and non-metastatic human breast cancer cell lines by immunohistochemical and TUNEL analysis. METHOD: MCF-7 and MDA-MB 231 cell lines were incubated under standard conditions forming Rapamycin and control groups. In immunohistochemical evaluation; mTOR pathway was evaluated with anti-IGF1, anti-PI3K, anti-pAKT1/2/3, anti-mTORC1, anti-mTORC2 and anti-ERK1 antibodies. The effect of apoptosis was also confirmed by TUNEL method. RESULTS: In this study, activation of PI3K/AKT/mTOR and related molecular pathways in the MDA-MB 231 and MCF-7 breast cancer cell line was evaluated and it was observed that these pathways could play a key role in cancer development. Increased apoptotic cells were observed in mTORC1 inhibition by Rapamycin administration. CONCLUSION: Targeting the mTOR pathway in breast cancer treatment may be a treatment option. In addition, the demonstration and confirmation of increased apoptosis in Rapamycin treated groups suggested that Rapamycin, an inhibitor of mTOR, is promising in the treatment of breast cancer (Tab. 2, Fig. 3, Ref. 66). Text in PDF www.elis.sk.
dc.identifier.issn0006-9248
dc.identifier.other1336-0345
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/8242
dc.language.isoEnglish
dc.publisherAEPRESS SRO
dc.subjectESTROGEN-RECEPTOR
dc.subjectMAMMALIAN PROTEIN
dc.subjectACTIVATION
dc.subjectINHIBITORS
dc.subjectCOMPLEX
dc.subjectTARGET
dc.subjectPHOSPHORYLATION
dc.subjectPI3K/AKT/MTOR
dc.subjectEXPRESSION
dc.subjectALPHA
dc.titleInvestigation of the effects of rapamycin on the mTOR pathway and apoptosis in metastatic and non-metastatic human breast cancer cell lines
dc.typeArticle

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