English

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Abstract

W B SAUNDERS CO-ELSEVIER INC

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Objective: Intraamniotic meconium has been responsible for intestinal damage in gastroschisis and meconiumdependent intestinal ischemia has been proposed to induce additional intestinal damage in gastroschisis. This study is aimed to determine the effects of lipid and water-soluble meconium subfractions on the contractility of the superior mesenteric artery (SMA). Materials and methods: The study was conducted on 18-day fertilized chick embryos (Gallus Domesticus). Meconium is fractioned into water and lipid-soluble components. Only one SMA tissue was prepared from each embryo and suspended in the organ bath. Isometric contraction responses (ICR) were created in SMA tissues by one hour of incubation in Krebs-Henseleit solution for each group. Groups consisted of control, meconium, water-soluble meconium subfraction and lipid-soluble meconium subfraction. ICR of the SMA specimens were evaluated with a transducer-amplifier system on a computer. The data were expressed (mean +/- 1SD) as milliNewton (mN). Results: The ICR of themeconium, water-solublemeconium subfraction and lipid-soluble meconium subfraction groupswere significantly highwhen compared to the control group (p < 0.01). Themeconiumandwater-soluble meconium subfraction created more contraction response than the lipid-soluble meconium subfraction (p < 0.01). The ICR of the meconium group was not different from the ICR of the water-soluble meconium subfraction group (p > 0.05). Conclusion: Water-solublemeconiumsubfraction has a profound vasoconstrictor effect on the SMA compared to the lipid-soluble meconium subfraction. (C) 2017 Elsevier Inc. All rights reserved.

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http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/6816

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