Evaluation of adjuvant activity of Astragaloside VII and its combination with different immunostimulating agents in Newcastle Disease vaccine

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dc.contributor.authorYakubogullari, N
dc.contributor.authorCoven, FO
dc.contributor.authorCebi, N
dc.contributor.authorCoven, F
dc.contributor.authorCoven, N
dc.contributor.authorGenc, R
dc.contributor.authorBedir, E
dc.contributor.authorNalbantsoy, A
dc.date.accessioned2024-07-18T12:05:54Z
dc.date.available2024-07-18T12:05:54Z
dc.description.abstractAstragaloside VII (AST-VII), a major cycloartane saponin isolated from Turkish Astragalus species, turned out to be one of the most active metabolites demonstrating Th1/Th2 balanced immune response. As Quillaja saponins are extensively used in adjuvant systems, this study made an attempt to improve AST-VII based adjuvant systems by using different immunostimulatory/delivery agents (monophosphoryllipid A (MPL), Astragalus polysaccharide (APS) and squalene) and to induce cellular and humoral immune response against a viral vaccine. For this purpose, Newcastle Disease vaccine (NDV) was chosen as a model vaccine. Swiss albino mice were immunized subcutaneously with LaSota vaccines in the presence/absence of AST-VII or developed adjuvant systems. AST-VII administration both in live/inactivated LaSota vaccines induced neutralizing and NDV specific IgG, IgG1 and IgG2b antibodies response as well as IL-2 and IL-4 production. APS based delivery systems enhanced the production of neutralizing antibody and the minor augmentation of IFN-? and IL-2 levels. Squalene emulsion (SE) alone or combined with AST-VII were effective in NDV restimulated splenocyte proliferation. As a conclusion, AST-VII and AST-VII containing adjuvant systems demonstrated Th1/Th2 balanced antibody and cellular immune responses in NDV vaccines. Thus, these systems could be developed as vaccine adjuvants in viral vaccines as alternative to saponin-based adjuvants.
dc.identifier.issn1045-1056
dc.identifier.other1095-8320
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/10067
dc.language.isoEnglish
dc.publisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
dc.subjectIN-VITRO
dc.subjectTRITERPENE GLYCOSIDES
dc.subjectSAPONINS
dc.subjectPOLYSACCHARIDES
dc.subjectROOTS
dc.subjectQS-21
dc.titleEvaluation of adjuvant activity of Astragaloside VII and its combination with different immunostimulating agents in Newcastle Disease vaccine
dc.typeArticle

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