Histopathological and ultrastructural effects of Losartan on embryonic rat kidney

Simple item page
dc.contributor.authorAkil, I
dc.contributor.authorInan, S
dc.contributor.authorGurcu, B
dc.contributor.authorNazikoglu, A
dc.contributor.authorOzbilgin, K
dc.contributor.authorMuftuoglu, S
dc.date.accessioned2024-07-18T12:02:31Z
dc.date.available2024-07-18T12:02:31Z
dc.description.abstractThe aim of our study was to investigate the histopathological, immunohistochemical and ultrastructural effects of Losartan (a selective angiotensin II type-1 receptor blocker) on renal development in rats. Twelve pregnant rats were divided into control and experimental groups. In the experimental group, Losartan (10mg/kg/day) was given via nasogastric tube, between the sixth day of implantation and time of sacrifice on embryonic days 18 and 20. All formalin-fixed, paraffin wax-embedded renal tissue sections were stained with hematoxylin and eosin or labelled for binding of primary antibodies against transforming growth factor-it (TGF-beta 1,-2,-3) using an avidin-biotin-peroxidase method. For electron microscopic examination, samples were fixed with glutaraldehyde and osmium tetroxide and embedded in araldite. Glomerular basement membrane (GBM) thickness was measured and compared using an unpaired t-test. Angiotensin II type-1 receptor antagonism by Losartan inhibited renal growth and delayed nephron maturation. Increased immunoreactivity of TGF-beta's was observed in developing nephron precursors and interstitial cells in the experimental group. Electron microscopical examination showed that thickening of the GBM was normal in the control group but an irregular thickening was seen in the experimental group (p < 0.001). It was also seen that epithelial cells of developing tubules underwent apoptosis in the experimental group. Thus, renal development in rats seems to depend on an intact renin-angiotensin system. (c) 2005 Elsevier GmbH. All rights reserved.
dc.identifier.issn0065-1281
dc.identifier.other1618-0372
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/8475
dc.language.isoEnglish
dc.publisherELSEVIER GMBH, URBAN & FISCHER VERLAG
dc.subjectRENIN-ANGIOTENSIN-SYSTEM
dc.subjectGROWTH-FACTOR-BETA
dc.subjectTGF-BETA
dc.subjectTRANSFORMING GROWTH-FACTOR-BETA-1
dc.subjectEXTRACELLULAR-MATRIX
dc.subjectCELL-DEATH
dc.subjectEXPRESSION
dc.subjectAPOPTOSIS
dc.titleHistopathological and ultrastructural effects of Losartan on embryonic rat kidney
dc.typeArticle

Files

Collections

Web of Science Koleksiyonu