Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species

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dc.contributor.authorIşın AKYAR
dc.contributor.authorCengiz ÇAVUŞOĞLU
dc.contributor.authorMeltem AYAS
dc.contributor.authorSüheyla SÜRÜCÜOĞLU
dc.contributor.authorZEYNEP ARZU İLKİ
dc.contributor.authorDeniz Ece KAYA
dc.contributor.authorYeşim BEŞLİ
dc.date.accessioned2024-07-24T09:13:33Z
dc.date.available2024-07-24T09:13:33Z
dc.date.issued2018
dc.description.abstractBackground/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way ofidentifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permitsearly, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy ofphenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) comparedto standard sequence analysis.Materials and methods: A total of 155 Mycobacterium clinical and external quality control isolates, comprising nontuberculousmycobacteria (NTM) (n = 95) and the Mycobacterium tuberculosis complex (MTC) (n = 60), were included in the study.Results: Identification by MBT ML4.0 was correctly performed in 100% of MTC and in 91% of NTM isolates. All of the MTC isolateswere correctly differentiated from NTM isolates.Conclusion: Based on our results, MBT ML4.0 may be used reliably to identify both NTM and MTC.
dc.identifier.DOI-ID10.3906/sag-1801-198
dc.identifier.issn1300-0144
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/25384
dc.language.isoeng
dc.subject[Fen > Tıp > Cerrahi]
dc.titleEvaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species
dc.typeAraştırma Makalesi

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