Acute responses of strength-related gene expressions to maximum strength and force sense acuity
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Date
2024
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Abstract
Background/aim: Although high muscle strength worsens the sense of force, it is unknown whether there is a relationship between this deterioration and the underlying molecular mechanisms. This study examined the relationship between decreased force sense (FS) acuity and strength-related gene expressions. Materials and methods: Maximal voluntary isometric contraction (MVIC) and FS (50% MVIC) tests were performed on the knee joints of twenty-two subjects. The expression analyses were evaluated by qRT-PCR in blood samples taken before, after MVIC, after 50% MVIC, and 15 min after the test. Results: MVIC and FS error values were significantly correlated with each other (r = .659, p = .001). The qRT-PCR analyses demonstrated that the expressed mRNAs of the interleukin 6 (IL-6), alpha-actinin 3 (ACTN3), angiotensin-converting enzyme (ACE), brain-derived neurotrophic factor (BDNF), and ciliary neurotrophic factor receptor (CNTFR) genes dramatically increased until 50% MVIC and subsequently decreased 15 min after the exercise (p <.05). The muscle-specific creatine kinase (CKMM), myosin light chain kinase (MLCK), and G-protein β3 subunit (GNB3) genes reached their peak expression levels 30 min after MVIC (p < .05). ACE and ACTN3 gene expression increased significantly in parallel with the increased FS error (p <.05). These gene expression fluctuations observed at 50% MVIC and after the rest could be related to changes in cellular metabolism leading to fatigue. Conclusion: The time points of gene expression levels during exercise need to be considered. The force acuity of those whose maximal force develops too much may deteriorate. © TÜBİTAK.
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Actinin , Adult , Brain-Derived Neurotrophic Factor , Female , Gene Expression , Humans , Interleukin-6 , Isometric Contraction , Knee Joint , Male , Muscle Strength , Muscle, Skeletal , Peptidyl-Dipeptidase A , Young Adult , alpha actinin 3 , brain derived neurotrophic factor , ciliary neurotrophic factor receptor , creatine kinase MM , dipeptidyl carboxypeptidase , guanine nucleotide binding protein beta subunit , interleukin 6 , myosin light chain kinase , actinin , interleukin 6 , adult , Article , blood sampling , body composition , cell metabolism , exercise , fatigue , female , force , force sense acuity , gene expression , gene expression level , genetic analysis , human , knee joint , knee ligament , male , muscle isometric contraction , muscle strength , physical activity , real time polymerase chain reaction , RNA extraction , strength related gene expression , visual feedback , young adult , gene expression , genetics , metabolism , muscle isometric contraction , physiology , skeletal muscle