SELECTED KINETIC PROPERTIES OF POLYPHENOL OXIDASE EXTRACTED FROM ROSMARINUS OFFICINALIS L.

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dc.contributor.authorAydemir, T
dc.date.accessioned2024-07-18T11:40:13Z
dc.date.available2024-07-18T11:40:13Z
dc.description.abstractPolyphenol oxidase from Rosmarinus officinalis L. (PPO, EC 1:14:18.1) was extracted and partially purified by using (NH4)(2)SO4 precipitation and dialysis. Stable and highly active PPO extracts were obtained using 0.5% (w/v) PEG and 0.5% (w/v) Triton X-100 in 0.1 M phosphate buffer 7.0. K-M values were found to be as 14.3 mM for catechol. Four isoenzymes of Rosemary PPO were detected by PAGE with DL-dopa substrate. The enzyme was strongly inhibited by dithiotreitol, sodium metasulfite, sodium thiosulfate, ascorbic acid, and L-cysteine. Metal ions Ca++, Mg++, and Mn++ were poor inhibitors of rosemary PPO at 10 Mm.
dc.identifier.issn1094-2912
dc.identifier.other1532-2386
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/2253
dc.language.isoEnglish
dc.publisherTAYLOR & FRANCIS INC
dc.subjectPARTIAL-PURIFICATION
dc.subjectPHYSICOCHEMICAL CHARACTERIZATION
dc.subjectINHIBITION
dc.subjectSULFITE
dc.subjectMUSHROOM
dc.subjectBANANA
dc.subjectPLANTS
dc.subjectSEEDS
dc.subjectACID
dc.titleSELECTED KINETIC PROPERTIES OF POLYPHENOL OXIDASE EXTRACTED FROM ROSMARINUS OFFICINALIS L.
dc.typeArticle

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