Association of platelet-activating factor acetylhydrolase gene polymorphism with premature coronary artery disease in Turkish patients
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Date
2006
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Abstract
Objective: Platelet-activating factor (PAF) is a phospholipid with multiple actions that is involved in inflammatory diseases as well as in atherogenesis. It is inactivated by a plasma enzyme, PAF-acetylhydrolase (PAF-AH). Deficiency of this enzyme in plasma is caused by a missense mutation in the gene (G994T). The aim of this study was to investigate association of this mutation with premature coronary artery disease (CAD). Methods: One hundred and fifteen unrelated Turkish patients with a diagnosis of premature CAD and 128 unrelated healthy subjects were enrolled in this study. Genotyping was performed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Results: The prevalence of the G994T mutation in the patients was 2.60 % (heterozygote), and 0 % in the controls. There was no significant difference in allele frequency and genotype distribution among the study groups. Conclusion: The G9943T mutation in the plasma PAF acetylhydrola se gene is not associated with premature CAD in Turkish subjects.
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1-Alkyl-2-acetylglycerophosphocholine Esterase , Case-Control Studies , Coronary Arteriosclerosis , European Continental Ancestry Group , Female , Humans , Male , Middle Aged , Mutation , Polymerase Chain Reaction , Polymorphism, Genetic , Polymorphism, Restriction Fragment Length , Prevalence , Turkey , 1 alkyl 2 acetylglycerophosphocholine esterase , cholesterol , DNA fragment , genomic DNA , high density lipoprotein cholesterol , low density lipoprotein cholesterol , triacylglycerol , adult , article , body mass , controlled study , coronary artery disease , coronary risk , diabetes mellitus , DNA polymorphism , family history , female , gene frequency , gene mutation , genetic association , genotype , heterozygosity , human , hypertension , major clinical study , male , polymerase chain reaction , prevalence , protein blood level , restriction fragment length polymorphism , smoking habit , Turkey (republic) , vascular disease