Synthesis of silver nanoparticles using Alpinia officinarum rhizome extract induces apoptosis through down-regulating Bcl-2 in human cancer cells
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2022
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Abstract
In this study, silver nanoparticles were synthesized using Alpinia officinarum rhizome extract via an eco-friendly green synthesis method. The silver nanoparticles (AO-AgNPs) were characterized by UV–Vis spectrometry, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and dynamic light scattering. Further, the cytotoxic and apoptotic effects of AO-AgNPs were investigated in human cancer cells with different tissue origins via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and flow cytometric analyses, respectively. The expression levels of anti-apoptotic Bcl-2 protein were evaluated via a real-time polymerase chain reaction. The synthesized AO-AgNPs induced a significant cytotoxic effect in all tested cancer cells but not in normal cells. AO-AgNPs induced the percentage of apoptotic cells and reduced the levels of anti-apoptotic Bcl-2 mRNA levels in cancer cells. These results demonstrate the potential application of AO-AgNPs in cancer treatment. © 2022, Akadémiai Kiadó Zrt.
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Alpinia , Antineoplastic Agents , Apoptosis , Bromides , Humans , Metal Nanoparticles , Neoplasms , Plant Extracts , Rhizome , RNA, Messenger , Silver , 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide , Alpinia officinarum extract , antineoplastic agent , messenger RNA , plant extract , protein bcl 2 , silver nanoparticle , unclassified drug , antineoplastic agent , bromide , messenger RNA , metal nanoparticle , plant extract , silver , Alpinia officinarum , apoptosis , apoptotic cell percentage , Article , controlled study , down regulation , drug cytotoxicity , drug synthesis , DU145 cell line , energy dispersive X ray spectroscopy , flow cytometry , H69AR cell line , human , human cell , MCF-7 cell line , mRNA expression level , MTT assay , photon correlation spectroscopy , protein expression , real time polymerase chain reaction , rhizome , scanning electron microscopy , ultraviolet visible spectroscopy , Alpinia , apoptosis , metabolism , neoplasm , rhizome