Synthesis of silver nanoparticles using Alpinia officinarum rhizome extract induces apoptosis through down-regulating Bcl-2 in human cancer cells

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dc.contributor.authorAtmaca H.
dc.contributor.authorÇamlı Pulat Ç.
dc.contributor.authorIlhan S.
dc.date.accessioned2024-07-22T08:04:00Z
dc.date.available2024-07-22T08:04:00Z
dc.date.issued2022
dc.description.abstractIn this study, silver nanoparticles were synthesized using Alpinia officinarum rhizome extract via an eco-friendly green synthesis method. The silver nanoparticles (AO-AgNPs) were characterized by UV–Vis spectrometry, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and dynamic light scattering. Further, the cytotoxic and apoptotic effects of AO-AgNPs were investigated in human cancer cells with different tissue origins via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and flow cytometric analyses, respectively. The expression levels of anti-apoptotic Bcl-2 protein were evaluated via a real-time polymerase chain reaction. The synthesized AO-AgNPs induced a significant cytotoxic effect in all tested cancer cells but not in normal cells. AO-AgNPs induced the percentage of apoptotic cells and reduced the levels of anti-apoptotic Bcl-2 mRNA levels in cancer cells. These results demonstrate the potential application of AO-AgNPs in cancer treatment. © 2022, Akadémiai Kiadó Zrt.
dc.identifier.DOI-ID10.1007/s42977-022-00132-5
dc.identifier.issn26768615
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12531
dc.language.isoEnglish
dc.publisherAkademiai Kiado ZRt.
dc.subjectAlpinia
dc.subjectAntineoplastic Agents
dc.subjectApoptosis
dc.subjectBromides
dc.subjectHumans
dc.subjectMetal Nanoparticles
dc.subjectNeoplasms
dc.subjectPlant Extracts
dc.subjectRhizome
dc.subjectRNA, Messenger
dc.subjectSilver
dc.subject3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide
dc.subjectAlpinia officinarum extract
dc.subjectantineoplastic agent
dc.subjectmessenger RNA
dc.subjectplant extract
dc.subjectprotein bcl 2
dc.subjectsilver nanoparticle
dc.subjectunclassified drug
dc.subjectantineoplastic agent
dc.subjectbromide
dc.subjectmessenger RNA
dc.subjectmetal nanoparticle
dc.subjectplant extract
dc.subjectsilver
dc.subjectAlpinia officinarum
dc.subjectapoptosis
dc.subjectapoptotic cell percentage
dc.subjectArticle
dc.subjectcontrolled study
dc.subjectdown regulation
dc.subjectdrug cytotoxicity
dc.subjectdrug synthesis
dc.subjectDU145 cell line
dc.subjectenergy dispersive X ray spectroscopy
dc.subjectflow cytometry
dc.subjectH69AR cell line
dc.subjecthuman
dc.subjecthuman cell
dc.subjectMCF-7 cell line
dc.subjectmRNA expression level
dc.subjectMTT assay
dc.subjectphoton correlation spectroscopy
dc.subjectprotein expression
dc.subjectreal time polymerase chain reaction
dc.subjectrhizome
dc.subjectscanning electron microscopy
dc.subjectultraviolet visible spectroscopy
dc.subjectAlpinia
dc.subjectapoptosis
dc.subjectmetabolism
dc.subjectneoplasm
dc.subjectrhizome
dc.titleSynthesis of silver nanoparticles using Alpinia officinarum rhizome extract induces apoptosis through down-regulating Bcl-2 in human cancer cells
dc.typeArticle

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