Evaluation of the effects of gonadotropin-relasing hormone antagonist (GnRH-ant) and agonist (GnRH-a) in the prevention of postoperative adhesion formation in a rat model with immunohistochemical analysis
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2011
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Abstract
Objective: To investigate the effects of GnRH antagonist (GnRH-ant) and agonist (GnRH-a) in the prevention of postoperative pelvic adhesions by a visual scoring system and immunohistochemical methods in a rat uterine horn model. Design: Controlled experimental animal study. Setting: Animal laboratory at an academic research environment. Animal(s): Twenty-one Wistar albino rats. Intervention(s): Rats were randomized into three groups. One week before the operation the rats received either GnRH-ant or GnRH-a or saline solution; they then underwent surgical laparotomy, and both uterine horns were traumatized by a scalpel. Three weeks later, all rats were sacrificed and extension and severity of the adhesions in each group were scored by a visual scoring system. Adhesion tissues were evaluated immunohistochemically for vitronectin and u-PAR. Main Outcome Measure(s): Scores of extend and severity of adhesions and staining of vitronectin and u-PAR. Result(s): The extent of adhesion scores were 1.85 ± 0.86, 0.78 ± 1.05, and 0.42 ± 0.64, and the severity of adhesion scores were 1.71 ± 0.91, 0.57 ± 0.85, 0.50 ± 0.75 for control, GnRH-ant, and GnRH-a groups, respectively. The extent and severity of adhesions were significantly lower in both GnRH-ant and GnRH-a groups when compared with the control group. Adhesion extent scores in the GnRH-a group were lower than in the GnRH-ant group, but this difference was not significant. vitronectin and u-PAR staining were significantly greater in both the GnRH-ant and GnRH-a groups than in the control group. Conclusion(s): GnRH-ant as well as GnRH-a reduced postoperative adhesion formation in a rat model. This finding was supported immunohistochemically by vitronectin and u-PAR staining. © 2011 American Society for Reproductive Medicine.
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Animals , Female , Fertility Agents, Female , Gonadotropin-Releasing Hormone , Hormone Antagonists , Immunohistochemistry , Leuprolide , Postoperative Complications , Rats , Rats, Wistar , Receptors, Urokinase Plasminogen Activator , Severity of Illness Index , Time Factors , Tissue Adhesions , Uterine Diseases , Uterus , Vitronectin , cetrorelix , leuprorelin , urokinase , vitronectin , animal cell , animal experiment , animal model , animal tissue , article , controlled study , disease severity , drug effect , female , immunohistochemistry , laparotomy , nonhuman , pelvic disease , postoperative complication , postoperative pelvic adhesion , priority journal , randomized controlled trial , rat , scoring system , visual scoring system